Baculovirus-mediated protein expression is a robust experimental technique for producing recombinant higher-eukaryotic proteins because it combines high yields with considerable post-translational modification capabilities. In this expression system, the determination of the titer of recombinant baculovirus stocks is important to achieve the correct multiplicity of infection for effective ampli...

The aryl hydrocarbon receptor (AHR) is a transcription factor which activates gene transcription by binding to its corresponding enhancer as the heterodimer, which is consisted of AHR and the aryl hydrocarbon receptor nuclear translocator (ARNT). Human AHR can be rather difficult to study, when compared among the AHR of other species, since it is relatively unstable and less sensitive to some l...

The baculovirus expression system has become established as a popular method for obtaining high levels of recombinant protein synthesis in a eukaryotic environment (5). In a number of cases, however, researchers have reported decreased product quality and yield resulting from proteolytic activity late in the course of infection (2,3,8,12). The enzyme most likely to cause this degradation is V-C...

Kus proteins and many related proteins possess the C-terminal motif C‘ys-AAX. where A is an aliphatic ;imino acid and X is itny iiniino acid. ‘I’his sequence directs ii prenyltransferiise. fh-nesyltransferase in the c;ise of ltas and geranylger;inyItransferase in most other ltas-related proteins. t o prenylate the cysteine residue 11-01. The nitture of the amino acid residue X appears t o deter...