Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions. Due to its tremendous progress in terms of speed, read length, throughput and cost, Next-Generation Sequencing (NGS) has been increasingly us...

Background: Self-directed learning (SDL) is considered a vital learning parameter in health professions studies. This dynamic technique helps the medical graduates to take initiative for solving their learning problems and become a lifelong learner to meet the challenges in health care profession. Methods: The 100 students of first year M.B.B.S were randomly divided into two batches of 50 stude...

We present an algorithm for fast stochastic gradient descent that uses a nonlinear adaptive momentum scheme to optimize the late time convergence rate. The algorithm makes eeective use of curvature information, requires only O(n) storage and computation, and delivers convergence rates close to the theoretical optimum. We demonstrate the technique on linear and large nonlinear back-prop networks...

Contents 1. Introduction 2. The P. pastoris expression system 2.1. The AOX1 promoter and alternative promoters 2.1.1 AOX1 promoter. 2.1.2. Strongly expressed alternative promoters. 2.1.3. Moderately expressed alternative promoters. 2.2. Host strains 2.2.1. Methanol utilization phenotype 2.2.2. Protease-deficient host strains 2.3. Expression vectors 3. Bioreactor process techniques for productio...

We derive in this paper the transient queue length and the busy period distributions of a single server queue with phase-type (PH) service times and a batch Markovian arrival process (BMAP). These distributions are obtained by the uniformization technique which gives simple recurrence relations. The main advantage of this technique is that it leads to stable algorithms for which the precision o...

We derive cost formulae for three di erent parallelisation techniques for training supervised networks. These formulae are parameterised by properties of the target computer architecture. It is therefore possible to decide the best match between parallel computer and training technique. One technique, exemplar parallelism, is far superior for almost all parallel computer architectures. Formulae...

MapReduce has recently emerged as a new paradigm for large-scale data analysis due to its high scalability, finegrained fault tolerance and easy programming model. Since different jobs often share similar work (e.g., several jobs scan the same input file or produce the same map output), there are many opportunities to optimize the performance for a batch of jobs. In this paper, we propose two n...

A technique was required for batch culturing and direct sensitivity testing of urine specimens to reduce increasing demands on laboratory services. A comparison was made between the filter paper strip method and multipoint inoculator technique using a Denley inoculator (Denley Ltd, Billingshurst, Sussex). The results showed that multipoint inoculation was a rapid, economical, and reliable metho...

Synthetic photochemistry carried out in classic batch reactors has, for over half a century, proved to be a powerful but under-utilised technique in general organic synthesis. Recent developments in flow photochemistry have the potential to allow this technique to be applied in a more mainstream setting. This review highlights the use of flow reactors in organic photochemistry, allowing a compa...