To understand the nature of recombination processes in Beauveria bassiana, double-auxotrophic complementary mutant strains were used to produce six heterokaryons by three different methods. Conidia from these heterokaryons were plated on selective media and stable haploid (but not diploid) recombinants were isolated. Single colony recombinants were recovered with both parental and non-parental ...

Entomopathogenic fungi are naturally occurring pathogens of some insects that produce enzymes capable of penetrating into an insect’s cuticle. Chitinases are thought to be responsible for entomopathogenic fungi’s ability to degrade cuticle. Primers were designed for the chi3 gene which encodes a chitinase CHIT30, from the mRNA transcript present in Metarhizium anisopliae. These primers were una...

The fungal entomopathogen Beauveria bassiana became established as an endophyte in in vitro-grown cocoa seedlings tested for up to 2 mo after inoculation to the radicle with B. bassiana suspensions. The fungus was recovered in culture from stems, leaves and roots. B. bassiana also was detected as an epiphyte 1 and 2 mo postinoculation. Penicillium oxalicum and five bacterial morphospecies also ...

The entomopathogenic fungus Beauveria bassiana (Balsamo 1835) Vuillemin is an effective alternative control agent against some agricultural pests and biological vectors of important diseases such as Chagas disease. In this work we studied an isolate of Beauveria bassiana from of the town of San Antonio Rayón, Puebla, Mexico and its entomopathogenic effects on Meccus pallidipennis (Stal 1872). P...

Recently the potential of enzymes for surface hydrophilisation and/or functionalisation of polyethyleneterephthalate (PET) and polyamide (PA) has been discovered. However, there was no correlation between enzyme class/activity (e.g. esterase, lipase, cutinase) and surface hydrolysis of these polymers and consequently no simple assay to estimate this capability. Enzymes active on the model subst...

To conduct laboratory experiments aimed at quantifying secondary acquisition of fungal conidia by western Xower thrips (Frankliniella occidentalis), an eYcient assay technique using Beauveria bassiana as the model fungus was developed. Various application protocols were tested and it was determined that the percent mortality did not vary among protocols. Peak mortality of secondinstar nymphs, u...

INTRODUCTION The use of entomopathogenic fungi to control disease vectors has become relevant because traditional chemical control methods have caused damage to the environment and led to the development of resistance among vectors. Thus, this study assessed the pathogenicity of entomopathogenic fungi in Triatoma dimidiata. METHODS Preparations of 108 conidia/ml of Gliocladium virens, Talarom...

Strains of hydrocarbon-degrading microorganisms (bacteria and fungi) were isolated from an agricultural soil in France. In a field, a portion was treated with oily cuttings resulting from the drilling of an onshore well. The cuttings which were spread at the rate of 600 g HC m-2 contained 10% of fuel oil hydrocarbons (HC). Another part of the field was left untreated. Three months after HC spre...