In serum-free mouse osteoblastic MC3T3-E1 cells, basic fibroblastic growth factor (bFGF) induced mRNA and protein for prostaglandin G/H synthase-2 (PGHS-2), the major enzyme in arachidonic acid (AA) conversion to prostaglandins. mRNA accumulation peaked at 1 h with bFGF 1 nM. In cells stably transfected with a 371-bp PGHS-2 promoter-luciferase reporter, bFGF stimulated luciferase activity, whic...

In the present study we investigated whether basic fibroblast growth factor (bFGF) plays a role in the proliferative response of smooth muscle cells (SMCs) to denuding injury. Rat carotid smooth muscle was found to express the mRNA for bFGF, and bFGF protein was found to be present in rat aorta by immunoblot analysis. Systemically administered bFGF was a potent mitogen for vascular SMCs in arte...

Biodegradable hydrogels with three different water contents were prepared through the glutaraldehyde crosslinking of gelatin with an isoelectric point of 5.0 under varied reaction conditions. The objective of this study is to investigate the effect of time period of basic fibroblast growth factor (bFGF) release that is modified by the hydrogel water content, on the bone regeneration. A bone fra...

Wound healing is a complex and dynamic process that includes multiple biological pathways has some successive periods. Most growth factor responsible for wound healing. Fibroblast positive effect on problems can be caused by diabetes. In the present study, we aimed to investigate exogenous bFGF supplementation serum TBARS, RSH NOx levels in hyperglysemic rats. Experiments were performed 30 male...

Background In vivo prevascularization followed by pedicled transfer has emerged as a promising strategy for tissue engineering in recent years. We recently demonstrated that capsule could serve novel axial vascular bed, although its high-density microvessels only be maintained about week. Objectives this present study, we aimed to demonstrate whether low-intensity pulsed ultrasound (LIPUS) prom...

PURPOSE To evaluate the abilities of recombinant adenovirus carrying the basic fibroblast growth factor (bFGF) gene to (1) produce bFGF protein in vitro and (2) rescue retinal photoreceptors in Royal College of Surgeons (RCS) rats in vivo. METHODS Cultured human retinal pigment epithelial cells were infected with one of the following two replication-deficient adenoviral vectors that drive ins...

Basic fibroblast growth factor (bFGF) and platelet-derived growth factor-BB (PDGFBB) modulate vascular wall cell function in vitro and angiogenesis in vivo. The aim of the current study was to determine how bovine aorta endothelial cells (BAECs) respond to the simultaneous exposure to PDGF-BB and bFGF. It was found that bFGF-dependent BAEC migration, proliferation, and differentiation into tube...

An experimental study examined the effect of basic fibroblast growth factor (bFGF) on the healing of corneal epithelial wounds in dogs. A corneal wound was made on one eye from each of 40 dogs with a corneal trephine (6 mm diameter). Four concentrations of bFGF (0, 0.1, 0.5, and 1.0 μg/ml) were applied to the affected eyes three times daily. Fluorescein staining was used to assess the closure o...

PURPOSE To assess the impact of basic fibroblast growth factor (bFGF) on photoreceptor function and morphology. METHODS Impact was assessed in two models. In one, the endogenous expression of bFGF in photoreceptors was raised by sectioning one optic nerve of rats 3 to 4 weeks before study. In the other, bFGF was injected into the vitreous chamber in rats and cats. Retinal function was assesse...

Basic fibroblast growth factor (bFGF) was immobilized to hydrogel scaffolds with retention of mitogenic and chemotactic activity. The bFGF was functionalized in order to incorporate it covalently within polyethylene glycol (PEG) hydrogel scaffolds by reaction with acryloyl-PEG-NHS. Hydrogels were formed by exposing aqueous solutions of PEG diacrylate, acryloyl-PEG-RGDS, and acryloyl-PEG-bFGF to...