The features of Helicobacter pylori adhesins, their interactions with their host counterparts, regulated and selective gene expressions are amongst the many clever strategies this microorganism undertakes to survive the otherwise sterile gastric milieu. The ingenious crafting of these interactions and the respective host reactions govern, in part, an array of consequences ranging from asympto...

We synthesized the first multifunctionalized phosphoinositide polyphosphate derivatives featuring a photo-removable protecting group (“cage”), photo-crosslinkable diazirine group, and terminal alkyne useful for click chemistry. demonstrate that lipid readily enter cells. After photo-crosslinking, cell fixation fluorescent tagging via chemistry, we determined intracellular location of before aft...

Understanding protein–ligand interactions in a cellular context is an important goal molecular biology and biochemistry, particularly for drug development. Investigators must demonstrate that drugs penetrate cells specifically bind their targets. Towards end, we present native mass spectrometry (MS)-based method analyzing uptake target engagement eukaryotic cells. This based on our previously i...

soils bacteria are frequently faced with various adverse environmental conditions and have developed a complex regulatory network to respond rapidly to environmental changes. in this study usefulness and applicability of stress proteins of bacteria which are produced after stress conditions were examined. the adaptation of soil bacteria involves the induction of stress proteins provide a nonspe...

During the attack of a pathogen, variety defense-associated proteins are released by host plant in apoplast to impede perceived attack. This study utilized mass spectrometry (LC-MS/MS) and label-free quantification method analyze apoplastic fluid (APF) from maize stalk identified responsive Fusarium verticillioides infection. We have 742 proteins, among these, 119 were differentially accumulate...

background and objectives: streptococcus agalactiae is the leading cause of bacterial sepsis and meningitis in newborns and results in pneumonia and bacteremia in adults. a number of s. agalactiae components are involved in colonization of target cells. destruction of peptidoglycan and division of covalently linked daughter cells is mediated by autolysins. in this study, autolytic activity and ...