Ryan K. Hamilton, Anna I Casasús, Ben Koopman, Spyros A. Svoronos Dept. Chemical Engineering, University of Florida, Gainesville, Florida 32611 Dept. Environmental Engineering Sciences, University of Florida, Gainesville, Florida 32611 © 2004 University of Florida Prepared for Presentation at the AIChE Annual Meeting / November 2004 / Session 10B10 Advances in Biochemical Processing: Control an...

The assembly of large recombinant DNA encoding a whole biochemical pathway or genome represents a significant challenge. Here, we report a new method, DNA assembler, which allows the assembly of an entire biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cerevisiae. We show that DNA assembler can rapidly assemble a functional D-xylose utilization pathway...

Metabolic engineering is generally defined as the redirection of one or more enzymatic reactions to produce new compounds in an organism, improve the production of existing compounds, or mediate the degradation of compounds. In highlighting progress in plant metabolic engineering over the past 25 years, it is first important to stress that it is in fact quite a young science in plants. Our know...

Metabolic engineering modifies cellular function to address various biochemical applications. Underlying metabolic engineering efforts are a host of tools and knowledge that are integrated to enable successful outcomes. Concurrent development of computational and experimental tools has enabled different approaches to metabolic engineering. One approach is to leverage knowledge and computational...

Research in systems biology focuses on the prediction and control of the behavior of biochemical networks through the use of mathematical models that accurately capture the responses of the network to certain types of perturbations. The construction of models based on the observations of these responses, referred to as reverse engineering, is an important step in discovering the structure and d...

Tissue engineering involves using the principles of biology, chemistry and engineering to design a 'neotissue' that augments a malfunctioning in vivo tissue. The main requirements for functional engineered tissue include reparative cellular components that proliferate on a biocompatible scaffold grown within a bioreactor that provides specific biochemical and physical signals to regulate cell d...

Switch like responses appear as common strategies in the regulation of cellular systems. Here we present a method to characterize bistable regimes in biochemical reaction networks that can be of use to both direct and reverse engineering of biological switches. In the design of a synthetic biological switch, it is important to study the capability for bistability of the underlying biochemical n...

Replacement of the knee meniscus requires a material possessing adequate geometrical and biomechanical properties. Meniscal tissue engineering attempts have been unable to produce tissue with collagen content and biomechanical properties, particularly tensile properties, mimicking native menisci. In an effort to obtain the geometric properties and the maturational growth necessary for the recap...

The kinetic models are fundamental knowledge about biochemical and microbial processes. The object of the work is the kinetic model researching based on the biochemical and microbial mechanisms of cell growth, substrate consumption and product formation. The ethanol biosynthesis process as fuel from renewable resources is the engineering goal. The engineering goal requires the inclusion of the ...