The interaction between semiconductor nanowires (NWs) and metallic nanocolloids were studied upon bioconjugation of two biomaterials: d-biotin and streptavidin. 5-fold intensity enhancement of luminescence and a blue shift of the emission peak were observed after conjugation with Au NPs. It can be interpreted in terms of the electromagnetic field enhancement effect in the presence of metallic N...

New synthetic methods to rapidly access useful fluorophores are needed to advance modern molecular imaging techniques. A new variant of the classical Smiles rearrangement is reported that enables the efficient synthesis of previously inaccessible C4'-O-alkyl heptamethine cyanines. The key reaction involves N- to O-transposition with selective electrophile incorporation on nitrogen. A representa...

Bioconjugation reactions are critical to the modification of peptides and proteins, permitting introduction biophysical probes onto proteins as well drugs for use in antibody-targeted medicines. A diverse set chemical reagents can be employed these circumstances covalently label protein side chains, such amine moiety chain lysine thiol functionality cysteine residues, two more frequently sites ...

The development of label-free biosensors with high sensitivity and specificity is of significant interest for medical diagnostics and environmental monitoring, where rapid and real-time detection of antigens, bacteria, viruses, etc., is necessary. Optical resonant devices, which have very high sensitivity resulting from their low optical loss, are uniquely suited to sensing applications. Howeve...

Cadmium-free silver-indium-sulfide (Ag-In-S or AIS) chalcopyrite quantum dots (QDs) as well as their core-shell structures (AIS/ZnS QDs) are being paid significant attention in biomedical applications because of their low toxicity and excellent optical properties. Here we report a simple and safe synthetic system to prepare high quality AIS and AIS/ZnS QDs using thermal decomposition. The synth...

Polymeric fibers have drawn recent interest for uses in biomedical technologies that span drug delivery, regenerative medicine, and wound-healing patches, amongst others. We have recently reported a new class of fibrous biomaterials fabricated using coextrusion and a photochemical modification procedure to introduce functional groups onto the fibers. In this report, we extend our methodology to...

Gaussia luciferase (Gluc)-with its many favorable traits such as small size, bright emission, and exceptional stability-has become a prominent reporter protein for a wide range of bioluminescence-based detection applications. The ten internal cysteine residues crucial to functional structure formation, however, make expression of high quantities of soluble protein in bacterial systems difficult...

Many in vivo and vitro applications using gold nanoparticles (AuNPs) require (i) their PEGylation, as it increases stability prevents nonspecific protein adsorption, (ii) conjugation to biomolecules, that provides them with specific recognition properties. Currently, the functionalization of AuNPs is based on thiol chemistry suffers from two major drawbacks: Au–S bond labile confers limited che...