Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying a synthetic "population control" circuit that regulates cell density through a feedback mechanism based on ...

Bacterial communities such as biofilms are widely recognized as being important for survival and persistence of bacteria in harsh environments. Mechanistic models of biofilm growth indicate that the way in which the surface is seeded can effect the morphology of simulated biofilms. Experimental studies indicate that genes which are important for chemotaxis also influence biofilm formation, perh...

Biofouling in pipelines is a function of the inner roughness of the specific piping material that is used in distribution systems and the concomitant biofilm formation. To test the effect of velocity on the growth of biofilm, a Roto-Scope was designed and built to imitate different materials and flow conditions in potable water distribution systems. Biofilm formation was monitored using DAPI st...

We propose that the rheological properties of background fluid play an important role in the interaction of microorganisms with the flow field. The viscoelastic-induced migration of microorganisms in a vortical flow leads to the emergence of a limit cycle. The shape and formation rate of patterns depend on motility, vorticity strength, and rheological properties of the background fluid. Given t...

some of pathogenic and food spoilage bacteria can attach on food contact surfaces and form a biofilm, the source of contamination of foods. biofilm is a functional consortium of microorganisms attached to the surface and is embedded in the extracellular polymeric substances (eps) produced by the microorganisms. biofilms due to special structure and eps are more resistant to antimicrobial agents...

Biofilm formation depends on several factors. The influence of different osmolarities on bacterial biofilm formation was studied. Two strains (Enterobacter sp. and Stenotrophomonas sp.) exhibited the most remarkable alterations. Biofilm formation is an important trait and its use has been associated to the protection of organisms against environmental stresses.

Article history: Bacterial biofilm formation Received 12 July 2008 Received in revised form 10 October 2008 Accepted 6 December 2008 Available online 24 December 2008