Hydrogen is a promising energy carrier of the future, nevertheless biohydrogen technologies are still in their infancy. If biohydrogen systems are to become commercially competitive, they must be able to synthesize hydrogen at rates that are sufficient to power fuel cells of a sufficient size to carry out practical work [1]. Before the concept of hydrogen economy becomes a reality, a safe, econ...

A gfp- and kanamycin-resistance gene-containing plasmid pUCGK was successfully constructed and transformed into Enterobacter aerogenes to develop a rapid GFP-based method for quantifying the bacterial concentration under anaerobic conditions for production of biohydrogen. Since the use of GFP as a molecular reporter is restricted by its requirement for oxygen in the development of the fluoropho...

The cell growth and product formation kinetics of anaerobic biohydrogen production by mixed anaerobic cultures was investigated using unstructured models in the present study. They described the realtionship between biomass growth and product formation in the hydrogen production process. Experimental results show that the Logistic model and LeudekingPiret model could be adopted to describe the ...

Conventional microbiology methods used to monitor microbial biofuels production are based on off-line analyses. The analyses are, unfortunately, insufficient for bioprocess optimization. Real time process control strategies, such as flow cytometry (FC), can be used to monitor bioprocess development (at-line) by providing single cell information that improves process model formulation and valida...

The need for renewable and sustainable fuel energy storage sources is pressing. Biohydrogen has the potential to be a storable carrier, direct diverse building block various downstream products. Utilizing microbial electrolysis cells (MECs) produce biohydrogen from residue streams, such as organic fraction of municipal solid waste (OFMSW), agricultural residues wastewater facilitate utilization...

As the energy demand is continuously rising with increase in population, use of fossil fuels also increasing at same rate. These release greenhouse gases (GHG) which are harmful to human health and our environmental these expected exhaust near future. This eventually has led an emerging need shift a more reliable, sustainable, clean source. Biohydrogen as fuel potential alternative, hydrogen pr...

Cyanobacterial mats are laminated microbial ecosystems which occur in highly diverse environments and which may provide a possible model for early life on Earth. Their ability to produce hydrogen also makes them of interest from a biotechnological and bioenergy perspective. Samples of an intertidal microbial mat from the Elkhorn Slough estuary in Monterey Bay, California, were transplanted to a...