INTRODUCTION The aim of this study was to detect the prevalence of the extended-spectrum beta-lactamase (ESBL)-encoding CTX-M gene in Escherichia coliisolates. METHODS Phenotypic screening of 376 E. coli isolates for ESBL was conducted using disk diffusion. ESBL-producing isolates were tested using PCR and specific primers. The bla(CTX-M) cluster was identified using the RFLP method, and its ...

Severe diabetic foot ulcers (DFUs) patients visiting Sir Sunderlal Hospital, Banaras Hindu University, Varanasi, were selected for this study. Bacteria were isolated from swab and deep tissue of 42 patients, for examining their prevalence and antibiotic sensitivity. DFUs of majority of the patients were found infected with Enterococcus spp. (47.61%), Escherichia coli (35.71%), Staphylococcus sp...

Objectives To evaluate a genome-based surveillance of all Danish third-generation cephalosporin-resistant Escherichia coli (3GC-R Ec ) from bloodstream infections between 2014 and 2015, focusing on horizontally transferable resistance mechanisms. Methods A collection of 552 3GC-R Ec isolates were whole-genome sequenced and characterized by using the batch uploader from the Center for Genomic ...

background and objectives: salmonella is an important food-borne pathogen in humans. strains of salmonella spp. that producing extended-spectrum β-lactamases have become a concern in medicine regarding both antimicrobial treatment and infection control program. the objective of this study was to describe the antibiotic susceptibility, esbl production and determining the prevalence of the bla ct...

Extended-spectrum β-lactamases (ESBLs) are the bacterial enzymes that make them resistant to advanced-generation cephalosporins. CTXM enzymes (the most prevalent ESBL-type) target cefotaxime. Aims of the study were: Modelling of CTX-M enzyme from bla(CTX-M) sequences of clinical Escherichia coli isolatesDocking of cefotaxime with modelled CTX-M enzymes to identify amino acid residues crucial to...

OBJECTIVES To assess the prevalence and types of genes encoding extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter spp. from Bolivia. METHODS A total of 642 clinical isolates were collected consecutively during a 4 month period (September to December 2004). Resistance or reduced susceptibility to cefotaxime and/or cef...

OBJECTIVES To investigate the presence of antibiotic resistance genes (ARGs), related to commonly used β-lactams and quinolones, in Escherichia coli present in hospital wastewater in central India. METHODS Cefotaxime- and ciprofloxacin-resistant E. coli isolates from hospital-associated wastewater samples were collected from two tertiary care hospitals in the Ujjain district of India during 2...

conclusions considering the high prevalence of esbl genes (bla ctx-m, 87%; and bla tem, 82%), fluoroquinolones may be used as an alternative drug in treatment, although resistance to this family is increasing as well. as a result, this increasing trend should be prevented using appropriate guidelines for prescription. background escherichia coli is the most important as well as the most common ...

(Figure S1, available as Supplementary data at JAC Online). These plasmids were reported not only in E. coli but also in S. enterica, in particular of serovars Llandoff, London, Newport and Typhimurium of chicken and human origin. 3 In conclusion, the bla CTX-M-1 IncI1/ST3 plasmid, which is dominant in various animal species in Europe and has also been reported in humans infected with S. enteri...