OBJECTIVES The multiresistance plasmid pKP96 from Klebsiella pneumoniae was sequenced completely and analysed concerning its genetic environment and distributing of antimicrobial resistance genes. METHODS The complete sequence of the plasmid was determined using a whole-genome shotgun approach. MICs of 13 antimicrobial agents were determined using Etests. A conjugation experiment was performe...

Shigellosis is a major source of gastroenteritis throughout the world (14). Extended-spectrum -lactamases (ESBLs), including cefotaximases (CTX-M), confer resistance to extendedspectrum cephalosporins and significantly compromise the treatment options for shigellosis. Numerous ESBLs have been described among Enterobacteriaceae (2, 8, 13); however, only a single CTX-M-producing Shigella isolate ...

Recent reports raised concerns about the role that farm stock may play in the dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria. This study characterized the ESBLs in two Escherichia coli and three Klebsiella pneumoniae subsp. pneumoniae isolates from cases of clinical bovine mastitis in the United Kingdom. Bacterial culture and sensitivity testing of bovine mastitic milk...

In this study, we attempted to isolate Escherichia coli from healthy adults in Ho Chi Minh City, Vietnam, and characterized its antimicrobial resistance profile, extended-spectrum β-lactamase (ESBL) genotype, phylogenetic grouping and virulence gene profile. A total of 103 E. coli isolates were obtained, and most of them were antimicrobial resistant such to streptomycin (80.6%), tetracycline (6...

Stenotrophomonas maltophilia is a widespread environmental microorganism (1) that has emerged as a significant opportunistic pathogen (2) due to its intrinsic resistance to almost all available antibiotics. Apart from native -lactamases L1 and L2 (1), acquired extended-spectrum -lactamases (ESBLs) were also identified (3, 4), pointing to S. maltophilia as a potential reservoir. Furthermore, res...

Background Due to increasing colistin usage, the dissemination of the colistin-resistant gene mcr-1 has been increasingly investigated. The aim of this study was to determine whether a traveler on a short-term international trip to a developing country could bring mcr-1 back to their home country. Materials and methods Thirty-four travel events from Japan to Vietnam encompassing 19 travelers ...

BACKGROUND The already high and increasing occurrence of extended-spectrum beta-lactamases (ESBL) producing Escherichia coli in European broiler populations is of concern due to the fact that third and fourth generation cephalosporins are deemed critically important in human medicine. In Sweden 34% of the broilers carry ESBL/pAmpC producing E. coli in their gut, despite the absence of a known s...

background and objective: organisms producing ctx-m β-lactamases are emerging as a source of resistance to oxyiminocefalosporins such as ceftriaxone and ceftazidime. however, the laboratory detection of these strains is not well defined. in this study, phenotypic assay for screening of extended-spectrum β-lactamases producing strains and molecular assay for the identification of ctx-m β-lactama...

Puerperal infection is a common complication during postnatal period in developing countries. Bacterial species, drug resistance, and genetic characteristics were investigated for a total of 470 isolates from puerperal infections in Bangladesh for a 2-year period (2010-2012). The most common species was Escherichia coli (n=98), followed by Enterococcus faecalis (n=54), Staphylococcus haemolytic...