Background: In this study we evaluate the effects of different doses of GDF9 with fixed dose of BMP15 on quality of in vitro maturated sheep oocytes. Materials and Methods: Good ovine Cumulus-oocyte complexes (COCs) were first transferred to Pre-IVM medium, and then to three IVM mediums supplemented with 10%v/v BMP15 and: 1) 175 ng/ml 2) 131 ng/ml or 3) 219 ng/ml GDF9. After maturation period, ...

This study aimed to examine the polymorphism of BMP15 (FecX$^{G}$ and FecX$^{I}$) GDF9 (G1 G4) genes association between genotypes litter size in Akkaraman breed. Blood samples were taken from 100 ewes total. Genomic DNA was obtained whole blood. The PCR-RFLP technique employed determine polymorphisms under investigation. G1 only polymorphic among four loci analyzed. frequencies for GA GG 0.26 ...

Most organisms modulate their reproductive activity responding to day length by the nocturnal release of melatonin by the pineal gland. This hormone is also responsible for synchronizing reproduction with specific external environment stimuli in order to optimize reproductive success.The aim of this study was to establish the effect of melatonin on zebrafish reproduction.Adult females were dail...

The onset of menopause has important implications on women's fertility and health. We previously identified genetic variants in genes involved in initial follicle recruitment as potential modifiers of age at natural menopause. The objective of this study was to extend our previous study, by searching for pairwise interactions between tagging single nucleotide polymorphisms (tSNPs) in the 5 gene...

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the families of tgf-β proteins are the most important growth factors in the ovary for growth and differentiation of early ovarian follicles. three related oocyte-derived members of the transforming growth factor-β superfamily namely growth differentiation factor 9 (gdf9), bmp15 and bmpr-ib have been shown to be essential for follicular growth and ovulation. different mutations in the gdf9 gene ...

CONTEXT Idiopathic primary ovarian insufficiency (POI) is a major cause of amenorrhea and infertility. POI affects 1% of women before age 40 years, and several genetic causes have been reported. To date, POI has been considered a monogenic disorder. OBJECTIVE The aim of this study was to identify novel gene variations and to investigate if individuals with POI harbor mutation in multiple loci...

Abstract Background Growth differentiation factor 9 ( GDF9 ) and bone morphogenetic protein 15 BMP15 genes play important roles in folliculogenesis. Altered expression of the two have been found among patients with poor ovarian response (POR). In this prospective cohort study, we determined follicle fluid (FF) granulosa cells (GCs) derived from responders grouped by age, explored its correlatio...

ObjectiveTo characterize and evaluate the variation in serum concentrations of oocyte-secreted growth differentiation factor 9 (GDF9) bone morphogenetic protein 15 (BMP15) throughout menstrual cycle women from young to advanced reproductive ages.DesignCross-sectional, observational, exploratory study.SettingMulticenter university-based clinical practices laboratories.Patient(s)Serum was collect...