The effects of lipid-rich bovine serum albumin (LR-BSA) on the development of porcine blastocysts produced in vitro were examined. Addition of 0.5 to 5 mg/ml LR-BSA to porcine blastocyst medium (PBM) from Day 5 (Day 0 = in vitro fertilization) significantly increased the hatching rates of blastocysts on Day 7 and the total cell numbers in Day-7 blastocysts. When Day-5 blastocysts were cultured ...

The abbreviations used are: ACN, acetonitrile; BSA, bovine serum albumin; DAPI, 4’,6’diamidino-2-phenylindole; DGF-1, dispersed gene family-1; DMEM, Dulbecco's Modified Eagle's Medium; dNTP, deoxynucleotide triphosphate; DTT, dithiothreitol; FITC-BSA, fluorescein isothiocyanate labelled bovine serum albumin; GST, glutathione S-transferase; HRP, horseradish peroxidase; IAA, Iodoacetamide; LIT, l...

Passage of bovine serum albumin (BSA) into unimplanted rabbit blastocysts was studied using intravenous or intrauterine injection of the mother and in vitro cultivation of 5-day blastocysts. BSA concentration in blastocyst fluid was measured using a quantitative radial immunodiffusion method and confirmed by double diffusion in agar. Intravenous injection of up to 200 mg/kg body weight produced...

Using UV-Vis spectrophotometric method the interaction of water soluble phthalocyanine, Cobalt(ΙΙ) 4,4′,4′′,4′′′- tetrasulfophthalocyanine(CoTSPc), with bovine serum albumin (BSA) to determine the formation constant and related thermodynamic functions. The measurements were considered in 1mM sodium phosphate buffer, pH 7.0 and at 5 different temperatures 20, 25, 30, 35 and 40ºC. The results sho...

The lipid A component of bacterial lipopolysaccharides (endotoxins), when complexed to bovine serum albumin (BSA) or human serum albumin (HSA), was shown to be a potent pyrogen. Furthermore, rabbits could be protected against endotoxin fever by immunization with both lipid A.BSA and lipid A.HSA complexes. The results presented in this paper show that lipid A is responsible for the pyrogenic act...

IgG antibodies to bovine serum albumin (BSA) were measured in 91 serum samples from children 4-17 years of age with newly diagnosed insulin-dependent diabetes mellitus (IDDM). A total of 80 healthy 12-year-old children served as control subjects. BSA antibody values > 3 SDs were obtained in 6 IDDM patients and 3 control subjects (P = 0.50), and values > 2 SDs were obtained in 12 IDDM patients a...

Diabetics have an increased prevalence of periodontitis, and diabetes is one of the causative factors of severe periodontitis. Apoptosis is thought to be involved in this pathogenic relationship. The aim of this study was to investigate apoptosis in human periodontal ligament (PDL) fibroblasts induced by advanced glycation end products (AGEs) and their receptor (RAGE). We examined the roles of ...

Extracellular vesicles (EV) awakened interest in the research on mesenchymal stromal cells by exploring their paracrine effects. However, many isolation protocols use bovine serum albumin (BSA) during the preparation of the EV. Therefore, we produced 'sham' BSA-EV and tested them in comparison to EV derived from mesenchymal stromal cells (MSC-EV). We found that BSA-EV did not express MSC-specif...

Sexing and centrifugation of spermatozoa is one way to improve the quality spermatozoa. This study aimed obtain right time for sexing with Bovine Serum Albumin media on morphology X-Y in Simmental cattle. was carried out from October 2021 November at Laboratory Animal Reproduction Health Breeding Center Superior Livestock Forage, Sembawa. The design used this a completely randomized (CRD) 3 tre...

conclusions the prepared antibody can be used for the development of an elisa kit to assay afm1 in milk and other biological fluids. background aflatoxins are the most extensively studied group of mycotoxins produced by molds, especially the aspergillus group, which are highly toxic to animals and humans. objectives since immunoassay is a simple and rapid method for the analysis of many toxic s...