Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is a very important disease of cauliflower (Brassica oleracea botrytis group) resulting into 10-50% yield losses every year. Since there is a dearth of availability of resistance to black rot disease in B. oleracea (C genome), therefore exploration of A and B genomes was inevitable as they have been reported to be potential reservo...

Canola (Brassica napus L., Brassicaceae) research in Virginia began in the mid 1980s. A concerted and focused cooperative effort was initiated, with USDA-CSREES-National Canola Research Program funds, in 1993 by Virginia’s two Land Grant Universities: Virginia State University and Virginia Tech. A major goal of this effort, until recently, has been establishment of canola as a domestic source o...

A protocol for the induction of site-directed deletions and insertions in the genome of Brassica carinata with CRISPR is described. The construct containing the Cas9 nuclease and the guide RNA (gRNA) was delivered by the hairy root transformation technique, and a successful transformation was monitored by GFP fluorescence. PAGE analysis of an amplified region, presumably containing the deletion...