AIM The aim was to assess the effects of fetal bovine serum (FBS) and estrus buffalo serum (EBS) on in vitro maturation rate of oocytes in buffalo. MATERIALS AND METHODS Maturation rate of oocytes was assessed in two maturation media supplemented with 20% FBS and EBS. Oocytes maturation rate was evaluated on the basis of cumulus cell expansion and extrusion of polar body after 24 h of in vitr...

OBJECTIVE The present study was conducted to investigate the seroprevalence of Toxoplasma gondii infection in water buffaloes. METHODS The research was conducted on 131 water buffaloes from Samsun and Afyon provinces. Serum samples of buffaloes were tested for toxoplasmosis with the Sabin Feldman Dye Test (SFDT), using live tachyzoites and methylene-blue dye. RESULTS 87.79% of buffaloes had...

Gene expression studies require appropriate normalization methods for proper evaluation of reference genes. To date, not many studies have been reported on the identification of suitable reference genes in buffaloes. The present study was undertaken to determine the panel of suitable reference genes in heat-stressed buffalo mammary epithelial cells (MECs). Briefly, MEC culture from buffalo mamm...

Toxoplasmosis has been reported to occur in several animals and humans causing different clinical manifestations. The study was conducted to determine the frequency of Toxoplasma gondii antibodies (IgG) in water buffalo (Bubalus bubalis) across farms in Trinidad using a latex agglutination test. Of a total of 333 water buffalo tested, 26 (7.8%) were seropositive for T. gondii antibodies. Seropo...

Malignant Catarrhal Fever (MCF), a fatal disease of a large number of different ruminants, is caused by infection with either a blue or black wildebeest-associated alcephaline herpesvirus 1 (AlHV-1) or the sheep-associated ovine herpes virus 2 (OvHV-2).Wildebeest and sheep are the natural hosts of these viruses respectively. Both these forms of MCF are of great economic importance, particularly...