نتایج جستجو برای: buthionine sulfoximine
تعداد نتایج: 1267 فیلتر نتایج به سال:
We have shown that cis diamminedichloroplatinum-(II) (DDP) resistant mutants can be isolated from the human ovarian carcinoma cell line A2780 using a single-step selection protocol with DDP. DDP resistant colonies were calculated to be present at a frequency of 1.7 x 10(-6)/viable cell using a fluctuation analysis. The mutational origin of these surviving colonies is inferred by the fact that t...
PURPOSE To characterize synthesis and transport of glutathione (GSH) by cultured human retinal pigment epithelial (RPE) cells. METHODS Cultured human RPE cells were depleted of glutathione, then incubated with various concentrations of cysteine, glutamate, and glycine or with glutathione. High-performance liquid chromatography was used to measure intracellular glutathione with time. RESULTS...
In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase-GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to ...
Ferroptosis is a unique variety of non-apoptotic cell death, driven by massive lipid oxidation in an iron-dependent manner. Since ferroptosis was introduced as concept 2012, it has demonstrated its essential role the pathogenesis neurodegenerative diseases and important therapy-resistant cancer cells. Thus, detailed molecular understanding both canonical alternative pathways required. There set...
This work is the next step in studying interplay between C-2028 (anticancer-active unsymmetrical bisacridine developed our group) and glutathione S-transferase/glutathione (GST/GSH) system. Here, we analyzed concentration- pH-dependent GSH conjugation of rat liver microsomes cytosol. We also applied three recombinant human GST isoenzymes, which altered expression was found various tumors. The f...
Cellular molecules possess various mechanisms in responding to oxidant stress. In terms of protein responses, protein S-glutathionylation is a unique post-translational modification of protein reactive cysteines forming disulfides with glutathione molecules. This modification has been proposed to play roles in antioxidant, regulatory and signaling in cells under oxidant stress. Recently, the in...
The biological basis for the general pharmacological resistance of human melanoma is unknown. A unique biochemical feature of the melanocyte is the synthesis of melanin, which leads to the generation of hydrogen peroxide and the consumption of reduced glutathione. This activity produces a state of chronic oxidative stress in these cells. We demonstrated previously that the expression of the c-j...
Tupling AR, Vigna C, Ford RJ, Tsuchiya SC, Graham DA, Denniss SG, Rush JW. Effects of buthionine sulfoximine treatment on diaphragm contractility and SR Ca pump function in rats. J Appl Physiol 103: 1921–1928, 2007. First published August 23, 2007; doi:10.1152/japplphysiol.00529.2007.—The purpose of this study was to examine the effects of glutathione (GSH) depletion and cellular oxidation on r...
Repeated cycles of vascular injury by benzo(a)pyrene (BaP) increase the onset and progression of atherosclerotic lesions in laboratory animals. This atherogenic response is partly mediated by activation of cis-acting antioxidant/electrophile response elements that enhance c-Ha-ras transcription in vascular smooth muscle cells (vSMCs). Activation of antioxidant/electrophile responsive cis-acting...
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