Biodiesel production from Waste Palm Cooking Oil (WPCO) is of interest to substitute fossil derived diesel fuel, due its renewable nature, cleaner emissions and non-toxic properties. Thus, in this study, biodiesel through transesterification process was optimized using immobilized lipase Candida rugosa WPCO collected the faculty’s cafeteria as a feedstock. Interaction between five operating fac...

Three new taxa, endemic of Sardinia, are described in the genus Asida Latreille, 1802: Asida (Asida) dryas sp. nov. (type locality: Capoterra, Gutturu Mannu), A. (Asida) anachoreta sp. nov. (type locality: Gonnosfanadiga, Punta Cammedda), and A. (Asida) solieri caroli ssp. nov. (type locality: Morgongiori, Is Benas). Additional systematic, distributional and ecological data are provided for som...

Abstract Candida rugosa lipase (CRL) is an enzyme that widely used in biopharmaceutical and biopesticide industries due to its high catalytic ability reusability. However, conditions from industrial processes are often outside CRL stability range may cause denaturation or destabilization of the enzyme, causing it be not reusable, hence lowering economic value. To increase activity, stability, r...

Abstract Lipase is an enzyme commonly applied as a catalyst in the food industry, synthesis of bioplastics, and production biodiesel. Candida rugosa lipase (CRL) one widely used lipases due to its high selectivity activity. However, operating temperatures can denature enzymes, affecting activity structural changes. An be immobilized on supporting matrix improve stability. Ethanol immobilization...

Research on lipase inhibitors could help in the therapy of diseases caused by lipase-producing microorganisms and in the design of novel lipase substrate specificities for biotechnology. Here we report a fast and sensitive colorimetric microassay that is low-cost and suitable for high-throughput experiments for the evaluation of lipase activity and inhibition. Comparison of Candida rugosa activ...

Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel fi...

The 17 non-universal serine codons (CTG) in the Candida rugosa LIP2 gene have been converted into universal serine codons (TCT) by overlap extension PCR-based multiple site-directed mutagenesis. An active recombinant LIP2 lipase was overexpressed in Pichia pastoris and secreted into the culture medium. The recombinant LIP2 showed distinguishing catalytic activities when compared with recombinan...