Combinatorial small molecule growth algorithm was used to design inhibitors for human carbonic anhydrase II. Two enantiomeric candidate molecules were predicted to bind with high potency (with R isomer binding stronger than S), but in two distinct conformations. The experiments verified that computational predictions concerning the binding affinities and the binding modes were correct for both ...

Davenport and Fisher (1938) reported the discovery DOGS P School of Medicine of large amounts of carbonic anhydrase in the gastric mucosa of cats, rats and rabbits. Davenport (1939) demonstrated that carbonic anhydrase is present in high concentration in the parietal cells and in lower concentration in the cells of the surface epithelium of the gastric mucosa of cats and rats. When those invest...

The carbonic anhydrase (CA: Carbonate hydrolyase: E.C.4.2.1.1) content from the leaves and flowers of mature yarrow (Achillea millefolium), a plant believed to possess healing qualities, was purified and characterized. The purification levels were 35.58 fold in the leaves and 40.21 fold in the flowers. The optimum temperatures were 25C and 40C for the leaves and flowers, respectively, and optim...

The distribution and biochemical diversity of neuroglial cells in the avian optic tectum were analyzed using immunohistochemical techniques. Polyclonal and monoclonal antibodies were employed to define the distributions and localizations of four proteins that are considered to be glial-specific in the mammalian central nervous system: glutamine synthetase, carbonic anhydrase-C, glial fibrillary...

An evaluation of several potential factors limiting carbon dioxide excretion by rainbow trout (Oncorhynchus mykiss) red blood cells was performed in vitro using a recently developed radioisotopic assay. Red blood cell (RBC) CO2 excretion was reduced by pretreatment (30min) of blood with the carbonic anhydrase inhibitor acetazolamide (final nominal concentration 1024 mol l21) or the Cl/HCO3 exch...

allura red (ar) is a widely used colorant in food industry, but there is debate on its potential security risk. in this study, in vitro inhibitory properties of the dye against carbonic anhydrase (ca) were evaluated. the esterase activity of purified ca decreased in the presence of ar, in a dose-dependent manner. regarding literature review and observed results, this preliminary study may provi...

PURPOSE To report the combination cocktail of strong steroid, non-steroidal anti-inflammatory drug (NSAID), and carbonic anhydrase inhibitor drops for treatment of cystoid macular edema. METHODS This is a retrospective case series of patients with cystoid macular edema managed with a topical combination of strong steroid (difluprednate), NSAID, and carbonic anhydrase inhibitor drops. The pati...