OBJECTIVE The immune response to post-translationally modified antigens is a key characteristic of rheumatoid arthritis. Carbamylation is such a posttranslational modification. Recently, we demonstrated that autoantibodies recognizing carbamylated proteins are present in sera of rheumatoid arthritis. The molecular mechanisms underlying the break of tolerance and hence the induction of anti-CarP...

Objectives Anti-carbamylated protein (anti-CarP) antibodies are detected in RA patients. Fetal calf serum is used as an antigen source in anti-CarP ELISA, and the precise target antigens have not been found. We aimed to identify the target antigens of anti-CarP antibodies. Methods Western blotting of anti-CarP antibodies was conducted. Anti-carbamylated human albumin (CarALB) antibody was det...

The common carp is a candidate model system for immunology research. Using next-generation sequencing technology, we have generated a huge amount of sequence reads from the carp genome and transcriptome. Currently, our aim is to identify carp genes involved in the development of the innate immune response, particularly TIR domain-containing genes, from a preliminary genome assembly. To achieve ...

The Capacitated Arc Routing Problem with Refill Points (CARP-RP) is a new variant of the Capacitated Arc Routing Problem (CARP). In a CARP situation, the vehicle servicing arcs has a finite capacity, and hence has to return to the depot or a secondary station to be refilled (or emptied). In contrast, in the CARP-RP, the vehicle servicing arcs must be refilled on the spot by using a second vehic...

To identify the molecular pathways that guide cardiac ventricular chamber specification, maturation and morphogenesis, we have sought to characterize factors that regulate the expression of the ventricular myosin light chain-2 gene, one of the earliest markers of ventricular regionalization during mammalian cardiogenesis. Previously, our laboratory identified a 28 bp HF-la/MEF-2 element in the ...

This study was designed to compare the activities of sarcoplasmic (SR) Ca2+-ATPase and Ca2+ uptake in fish and mammalian hearts and to determine whether thermal acclimation has any effect on the function of the cardiac SR in fish. To this end, we measured thapsigargin-sensitive Ca2+-ATPase activity and thapsigargin-inhibitable Ca2+ uptake velocity in crude cardiac homogenates of newborn and adu...

The Capacitated Arc Routing Problem (CARP) is a combinatorial optimization problem, which requires the identification of such route plans on given graph to number vehicles that generates least total cost. Dynamic CARP (DCARP) variation considers dynamic changes in problem. Artificial Bee Colony (ABC) algorithm an evolutionary was proven be able provide better performance than many other algorit...

See article by Zolk et al. [11] (pages 563–572) in this analogy with re-expression of foetal genes in several issue. cardiac pathologies, it was logical to assess CARP expresDespite several major advances in the management of sion in similar situations. Thus Eschenhagen’s group [8] congestive heart failure such as the introduction of andemonstrated that CARP expression, at the mRNA and giotensi...

The protein nucleolin, functionally involved in the main steps of ribosome biogenesis, is codified by a single copy gene in mammals. Here we report that at least three different genes codify for this protein in carp fish (Cyprinus carpio). This is the first description of the genomic organization of nucleolin in a teleost. The carp nucleolin gene includes 8.8 kb and contains 16 exons. Promoter ...

Introduction Grass carp (Ctenopharyngodon idellus) is an important species of freshwater fish in China. It accounts for about 20% of the total freshwater fishery production. Hemorrhagic disease of grass carp is the most serious infectious disease of grass carp and causes significant losses of fingerlings during rearing. Survival to market size is less than 20% if this disease occurs. Hemorrhagi...