Pressurized liquid extraction (PLE) has been used for the extraction of beta-sitosterol from food samples. In the present work, extractions were performed by mixing ground samples with Hydromatrix celite with methanol at 50 degrees C and 110 bar, using two 5-min cycles. After extraction, beta-sitosterol was analyzed directly by liquid chromatography with diode array detection. The extraction re...

Factor XII initiates the intrinsic coagulation cascade and may affect the fibrinolytic system. Routine coagulation tests used during cardiopulmonary bypass (CPB) are abnormal in factor-XII-deficient patients and are useless for monitoring anticoagulation in these patients. A factor-XII-deficient patient requiring CPB is described. The baseline celite activated clotting time (ACT) was greater th...

A protein fraction which inhibits growth of weanling rats was obtained from Phaseolus vulgaris seeds of the Sanilac variety by extraction with sodium chloride solution and removal of extraneous materials by precipitation at pH 4.0 and absorption on I : I bentonite-Celite. The protein was precipitated by 0.75 saturation with ammonium sulfate. The growth inhibiting protein fraction contained at l...

We applied various prepurification protocols (extraction with different solvents, liquid/solid separation on bonded silica media, Celite, and Sephadex LH20 chromatography) with a range of commercially available RIA kits to measure cortisol in urine samples. We then compared the results with the concentrations measured by a HPLC method validated with reference to isotope dilution gas chromatogra...

A large scale isolation of a disaccharide from a dialyzable fraction cf Micrococcus Zysodeikticus cell wall is described. This fraction was obtained by degradation of isolated walls with egg white lysozyme. After purification by chromatography on charcoal-Celite 2nd Dowex 1, the compound was characterized by a crystalline heptaacetyl methyl ester. Comparison with synthetic 0-2-acetamido-2-deoxy...

This paper describes a new method for qualitative and quantitative assay of abscisic acid and other acidic plant hormones, such as indoleacetic acid and the gibberellins, by the gas-liquid chromatography of their trimethylsilyl derivatives. Interfering substances in plant extracts were largely removed by preliminary column chromatography with carbon-celite and elution of the abscisic acid with ...

Estrone sulfate is quantitatively the most important estrogen in plasma. A method for its determination in human plasma is described, and the precision, accuracy, sensitivity, and specificity are defined. Free steroids were extracted from plasma with diethyl ether and steroid sulfates were isolated with use of Vlitos' reagent (methylene blue in dilute H2SO4/Na2SO4 solution). After enzymic hydro...