imprinted expression of most, though not all, known imprinted genes (reviewed in Tilghman, 1999). Differentially methylated regions have also been consistently Edinburgh EH4 2XU correlated with imprinted loci. On mouse chromosome United Kingdom 7, the region upstream of H19 (the imprinting control region—ICR) is methylated only on the paternally derived allele. It is now clear that the ICR orch...

Molecular imprinted Polymers (MIP) targeted for Tobacco mosaic virus (TMV) have been synthesized. Batch equilibrium studies using imprinted and non-imprinted polymer hydrogels in TMV and TNV solutions were conducted to determine virus-binding capacities. TMV-imprinted hydrogels showed increased binding to TMV (8.8 mg TMV/gpolymer) compared to non-imprinted hydrogels (4.2 mg TMV/gpolymer). Furth...

Phenylketonuria (PKU) is a critical disease in the disorder of amino acid metabolism, which mostly emerges children. The most common method used PKU treatment detection l-phenylalanine (PHEA) as biomarker PKU. In present work, new molecularly imprinted electrochemical electrode based on WS2 nanoflowers (NFs) N,B-doped graphene (WS2 NFs/N,B-GR) presented for PHEA assay milk samples. First all, N...

Cattle are the species used most frequently for the development of assisted reproductive technologies, such as nuclear transfer. Cattle cloning can be performed by a large number of laboratories around the world, and the efficiency of nuclear transfer in cattle is the highest among all species in which successful cloning has been achieved. However, an understanding of the expression of imprinte...

Transient neonatal diabetes (TND) is a rare but distinct type of diabetes. Classically, neonates present with growth retardation and diabetes in the first week of life. Apparent remission occurs by 3 months but there is a tendency for children to develop diabetes in later life. Evidence suggests it is the result of overexpression of an imprinted and paternally expressed gene/s within the TND cr...

Imprinting is a flexible and straightforward technique to generate selective sensor materials e.g. for mass-sensitive detection. Inherently, the strategy suits both molecular analytes and entire micro organisms or cells. Imprinted polyurethanes e.g. are capable of distinguishing the different xylene isomers with very appreciable selectivity factors. Combining imprinted titanates with surface tr...

In this study, solid state fermentation (SSF) was carried out to produce single cell protein (SCP) from sugarcane bagasse using Saccharomyces cerevisiae. The SSF experiment were performed in a tray bioreactor. The influence of several parameters including extraction buffer, initial moisture content of substrate, fermentation time, relative humidity in bioreactor, the bioreactor temperature and ...

Amitriptyline hydrochloride is a highly permeable active pharmaceutical ingredient (API). The function of these drugs is to block the reuptake of the neurotransmitters, norepinephrine and serotonin in the central nervous system. The nano-sized Amitriptyline (AT) imprinted polymer particles were synthesized successfully. The nanoparticles were characterized by Fourier transform infrared spectros...

Genomic imprinting leads to preferred expression of either the maternal or paternal alleles of a subset of genes. Imprinting is essential for mammalian development, and its deregulation causes many diseases. However, the functional relevance of imprinting at the cellular level is poorly understood for most imprinted genes. We used mosaic analysis with double markers (MADM) in mice to create uni...

We isolated mutations in Arabidopsis to understand how the female gametophyte controls embryo and endosperm development. For the DEMETER (DME) gene, seed viability depends only on the maternal allele. DME encodes a large protein with DNA glycosylase and nuclear localization domains. DME is expressed primarily in the central cell of the female gametophyte, the progenitor of the endosperm. DME is...