Background: Artificial oligonucleotides like DNA or RNA aptamers can be used as biodiagnostic alternatives for antibodies to detect pathogens. Comparing to antibodies, artificial oligonucleotides are produced easily at lower costs and are more stable. Neisseria meningitidis, the causative agent of meningitis, is responsible for about 1% of infections in an epidemic period. Specific DNA aptamers...

Aptamers have emerged as promising molecular probes for disease diagnosis and therapy. In the present study, the entire live cell-SELEX method was used to generate gastric cancer cell‑specific aptamers. Human gastric carcinoma AGS cells were used as target cells for positive selections and human normal gastric epithelial GES-1 cells as the negative cells for counter selections. The selection pr...

In this study, single-stranded DNA aptamers that switch structural conformation upon binding to the salivary peptide histatin 3 have been reported for first time. Histatin is an antimicrobial possesses capability of being a therapeutic agent against oral candidiasis and has recently linked as novel biomarker acute stress. The were identified through library immobilization version iterative in v...

Aptamers are promising gene components that can be used for the construction of synthetic gene circuits. In this study, we isolated an RNA aptamer that specifically inhibits transcription of T7 RNA polymerase (RNAP). The 38-nucleotide aptamer, which was a shortened variant of an initial SELEX isolate, showed moderate inhibitory activity. By stepwise doped-SELEX, we isolated evolved variants wit...

We study effects of the intrinsic charm on the hadronic production of Ξcc at SELEX, Tevatron and LHC. By taking the intrinsic charm into account, the cross-section of the production at SELEX is changed significantly. In contrast, at LHC and Tevatron the change is small because the gluon-gluon fusion becomes dominant. The measured cross-section at SELEX is much larger than the prediction based o...

We report a precise measurement of the D s meson lifetime. The data were taken by the SELEX experiment (E781) spectrometer using 600 GeV/c Σ, π and p beams. The measurement has been done using 918 reconstructed D s . The lifetime of the D s is measured to be 472.5±17.2±6.6 fs, using K (892)K and φπ decay modes. The lifetime ratio of D s to D 0 is 1.145 ± 0.049.

Aptamers are short nucleic acids that bind to defined targets with high affinity and specificity. The first aptamers have been selected about two decades ago by an in vitro process named SELEX (systematic evolution of ligands by exponential enrichment). Since then, numerous aptamers with specificities for a variety of targets from small molecules to proteins or even whole cells have been select...