The research included isolation and purification of Trypsin from serum patient with pancreatic cancer, estimation its molecular weight by using the two techniques gel filtration (using sephadex G-50) electrophoresis SDS). Therefore; it was noted that proteinous peak isolated ammonium sulfate solution is saturated (65%) had apparent (27000) Dalton. study (40) patients pancreas cancer their ages ...

Diagnostic algorithms for Wilson's disease (WD) recommend the determination of serum ceruloplasmin in addition to the slit-lamp examination required to identify Kayser–Fleischer rings as clues in order to decide on or decline further testing, while measurements of total serum copper and/or ceruloplasmin-unbound (“free”) copper are of value in monitoring pharmacotherapy [1,2]. The direct measure...

We describe a method for calculating the absorptivity (in terms of substrate consumed) of the colored solution obtained when o-dianisidine dihydrochloride is oxidized by ceruloplasmin. By oxidizing o-dianisidine dihydrochloride with known amounts of hydrogen peroxide we could determine that the molar reacting ratio of o-dianisidine to hydrogen peroxide is 2:1. So calculated, absorptivity is 9.6...

An analysis of the genome sequence database revealed novel types of two-domain multi-copper oxidases. The two-domain proteins have the conspicuous combination of blue-copper and inter-domain trinuclear copper binding residues, which is common in ceruloplasmin and ascorbate oxidase but not in nitrite reductase, and therefore are considered to retain the characteristics of the plausible ancestral...

Immersing Siberian sturgeon fry of an average body mass of 40 ± 4 g in epin solution (0.1 mg l -1 ) resulted in an increase of ceruloplasmin level over the entire experimental period (P < 0.001). The differences between the treated and control groups gradually increased reaching the maximum in the fourth and fifth weeks of the study when average levels of ceruloplasmin in the epin-treated group...