A simple method for viral DNA extraction using chelex resin was developed. The method used was eco-friendly and cost effective compared to other methods such as phenol chloroform method which use health hazardous organic reagents. Further, a polymerase chain reaction (PCR) based detection of canine parvovirus (CPV) using primers from conserved region of VP2 gene was developed. To increase the s...

Nucleic acid amplification technologies offer great promise for the rapid, sensitive and specific diagnosis of tuberculosis. However, the isolation of inhibitor-free DNA from biological specimens is a bottleneck of the PCR assay. Here we describe a simple method for the isolation of PCR-amplifiable DNA of Mycobacterium tuberculosis from all types of samples of pulmonary and extrapulmonary origi...

A method integrating a long waveguide capillary cell with a preconcentration resin in a multi-syringe flow injection analysis (MSFIA) system for iron determination in waters was developed. The determination of iron is based on a colorimetric reaction and two reagents were tested, ferrozine and ammonium thiocyanate. A liquid waveguide capillary cell (1.0 m pathlength, 550 μm i.d. and 250 μL inte...

The ascorbate oxidase activity of ceruloplasmin has been a subject of controversy because of (a) the relatively low oxidase activity of this serum copper protein toward ascorbic acid and (b) the different conclusions reached by several investigators. In 1951, Holmberg and Laurel1 (1) reported that ascorbic acid was a substrate of ceruloplasmin although it was less active as a substrate than p-p...

Objective: To determine an association of single nucleotide polymorphism the NRF2 gene (RS6721961) with semen parameters primary male infertility patients in Pakistani population.
 Study Design: Comparative cross-sectional study.
 Place and Duration Study: Department Biochemistry, Islamic International Medical College, Rawalpindi, collaboration two private clinics (American Infertilit...

The development of an in-line digestion system based on simple UV lamp is reported. The effect of photolysis on the preconcentration of copper was investigated using an in-line Chelex-l00 ion-exchange column linked to an atomic absorption spectrometer. Three model ligands, glycine, NTA and EDTA, have been used to demonstrate the effect of the digestion system. In a stopped-flow mode, over 90% o...