The p12 protein of murine leukemia virus (MuLV) group-specific antigen (Gag) is associated with the preintegration complex, and mutants of p12 (PM14) show defects in nuclear entry or retention. Here we show that p12 proteins engineered to encode peptide sequences derived from known viral tethering proteins can direct chromatin binding during the early phase of viral replication and rescue a let...

background: parathyroid hormone is an 84-amino acid peptide secreted by the parathyroid glands. its physiological role is maintenance of normal serum calcium level and bone remodeling. biological activity of this hormone is related to n-terminal 1-34 amino acids. the recombinant form of hormone (1-34) has been approved for treatment of osteoporosis from 2002. in this study, a novel fusion partn...

We describe the catalytic activity of the first chimeric ligase containing a foldameric sequence of β- and γ-amino acids. The chimeric backbone provides for the spatial arrangement of all functional groups involved in the formation of the catalytic site to allow efficient catalysis to take place. Our finding indicates significant progress in the field of functionally active artificial motifs.

The recombinant Kv7.2 calmodulin (CaM) binding site (Q2AB CaMBD) shows a high tendency to aggregate, thus complicating biochemical and structural studies. To facilitate these studies we have conceived bis-PEG-peptide CaMBD-mimetics linking helices A and B in single, easy to handle molecules. Short PEG chains were selected as spacers between the two peptide molecules, and a Cu(i)-catalyzed cyclo...

SEC72 encodes the 23-kD subunit of the Sec63p complex, an integral ER membrane protein complex that is required for translocation of presecretory proteins into the ER of Saccharomyces cerevisiae. DNA sequence analysis of SEC72 predicts a 21.6-kD protein with neither a signal peptide nor any transmembrane domains. Antibodies directed against a carboxyl-terminal peptide of Sec72p were used to con...

Synthetic peptides representing T- and B-cell epitopes from the fusion (F) protein of measles virus (MV) were tested for their ability to induce a protective immune response against intracerebral challenge with neuroadapted strains of MV and canine distemper virus (CDV) in mice. Of the panel of peptides tested, only a chimeric peptide consisting of two copies of a promiscuous T-cell epitope (re...

background: prostate cancer after lung cancer is the second cause of cancer-associated death in men. in recent years, targeted therapy of cancer has attracted researchers attention. the targeted therapy lead to decrease in side effects of drugs. studies have indicated that targeting peptides for cancer cells represent valuable tools for diagnostic and therapeutic. recently, phage display peptid...

milk proteins provide a natural source of bioactive peptides with potential health benefits and applications in the food industry. the release of these peptides from milk proteins is achieved either by hydrolysis using digestive proteases or by lactic acid bacteria fermentation. peptides, particularly those derived from milk proteins, can exert a wide range of nutritional, functional and biolog...

background and objective: enterohemorrhagic escherichia coli (ehec) o157:h7 is an important enteric pathogen in human causing bloody or nonbloody diarrhea, which may be complicated by hemolytic uremic syndrome (hus). cattle are an important reservoir of ehec. this research aims at vaccination with a divalent chimer protein composed of espa 120 and intimin 282 and its preventive effect of ehec o...

background and objectives: the linear epitopes of gp46-i, gp46-ii, gp21 and p19 are used in diagnosis of htlv-i/-ii infections. the aims of this study was to obtain high-level expression and purification of recombinant antigen (ra) containing these epitopes. large-scale preparation of such antigen probably worths for diagnostic purpose. materials and methods: the synthetic dna encoding ra was s...