The 45-kilobase tetracycline resistance plasmids pMAK175 and pUA466 from Campylobacter jejuni were examined using curing and mating experiments. However, these plasmids encoded neither cytotoxin production, as determined in Vero cells, nor enterotoxin activity, as determined in Chinese hamster ovary cells.

Abbreviations: CPRG, chlorophenol red-β-D-galactopyranoside; CFP, Cyan Fluorescent Protein; CHO, Chinese Hamster Ovary cells; FRET, Fluorescence Resonance Energy Transfer; GFP, Green Fluorescent Protein; HEX, hexachloro-carboxyfluorescein; HUVEC, Human Umbilical Vein Endothelial Cells; RHD, Rel Homology Domain; TET, tetrachloro-carboxyfluorescein; β-TrCP, beta-transducin repeat-containing prote...

The relationship between cell killing and the binding of the anticancer drug cis-diamminedichloroplatinum(II) (cis-DDP) to DNA was studied in six mammalian cell lines. Two of the human cell lines (COV413B) were of the same origin, comprising one sensitive to cis-DDP and the other with induced resistance to the drug. The four other lines, two rodent (RIF-1, Chinese hamster ovary) and two human (...

The relationship between cell killing and the binding of the anticancer drug m-diamminedichloroplatinum(II) (c/'s-DDP) to DNA was studied in six mammalian cell lines. Two of the human cell lines (COV413B) were of the same origin, comprising one sensitive to m-DDP and the other with induced resistance to the drug. The four other lines, two rodent (RIF-1, Chinese hamster ovary) and two human (A27...

Chinese hamster ovary cells were exposed to the sulfhydryl compound cysteamine at different temperatures (5 degrees C, 37 degrees C, 44 degrees C) at concentrations known to generate activated oxygen species. At 37 degrees C, the cellular glutathione (GSH) content increased linearly over the time of drug exposure (2 h) as compared to untreated cells or to cells kept at 5 degrees C during drug t...

Abbreviations: CPRG, chlorophenol red-β-D-galactopyranoside; CFP, Cyan Fluorescent Protein; CHO, Chinese Hamster Ovary cells; FRET, Fluorescence Resonance Energy Transfer; GFP, Green Fluorescent Protein; HEX, hexachloro-carboxyfluorescein; HUVEC, Human Umbilical Vein Endothelial Cells; RHD, Rel Homology Domain; TET, tetrachloro-carboxyfluorescein; β-TrCP, beta-transducin repeat-containing prote...

Kampo formulations are traditional herbal medications used in China and Japan for many centuries to treat diarrheal diseases such as cholera. Our studies were undertaken to identify and verify by chemical synthesis the active components that inhibited cholera toxin (CT), the virulence factor secreted by Vibrio cholerae, the causative agent of cholera. The Kampo formulation, Daio-kanzo-to, inhib...

Mitomycin C (MMC), a bifunctional alkylating agent, requires metabolic reduction to become biologically active. We have identified a series of genetically related Chinese hamster ovary cell lines which span approximately three orders of magnitude in the concentration of MMC required for cell killing. Many mechanisms, including drug transport, drug activation, drug detoxification, and the elimin...

Fringe modulates Notch signaling resulting in the establishment of compartmental boundaries in developing organisms. Fringe is a beta 3N-acetylglucosaminyltransferase (beta 3GlcNAcT) that transfers GlcNAc to O-fucose in epidermal growth factor-like repeats of Notch. Here we use five different Chinese hamster ovary cell glycosylation mutants to identify a key aspect of the mechanism of fringe ac...