Recombinant high density lipoprotein (rHDL) particles were prepared by cosonication of purified lipids and human apoproteins and incubated with partly purified cholesteryl ester transfer protein (CETP) and low density lipoprotein (LDL) containing [3H]cholesteryl ester. Increasing the triglyceride content relative to cholesteryl ester in rHDL significantly decreased the ability of the particles ...

The aim of this study was to evaluate the effect of a low-saturated-fat, low-cholesterol diet on plasma lipopoproteins, pre beta-high density lipoprotein (HDL) formation, lecithin:cholesterol acyltransferase (LCAT), cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP) activities, as well as on the ability of plasma to stimulate cellular cholesterol efflux. Twelve m...

Cholesteryl ester transfer protein (CETP) mediates the exchange of phospholipids and neutral lipids between the plasma lipoproteins, and plays an important role in high density lipoprotein (HDL) metabolism. While there are reports of low-level CETP secretion from cultured cells, the lack of a good model cell line has hampered the detailed study of CETP regulation and secretion. In this study, w...

This thesis is based on the following original articles, which are referred to in the text by their Roman numerals. acid content of LDL and lipoprotein metabolism in combined hyperlipidemia and primary moderate hypercholesterolemia. acid content of LDL and its relation to lipid concentrations and metabolism of LDL and cholesterol. Submitted. increases the sialic acid content of LDL in hyperchol...

Cardiovascular disease (CVD) remains a major burden for morbidity and mortality in the general population, despite current efficacious low-density lipoprotein-cholesterol-lowering therapies. Consequently, novel therapies are required to reduce this residual risk. Prospective epidemiological studies have shown that high-density lipoprotein-cholesterol (HDL-C) levels are inversely correlated with...

To determine whether nonhuman primates demonstrate the same alterations in transport of cholesteryl ester (CE) in plasma observed in diabetic humans, cholesteryl ester transfer (CET) was measured in cynomolgus monkeys with chronic spontaneous diabetes mellitus (glycated hemoglobin: diabetics 10.7 +/- 4.1%; controls 3.8 +/- 0.8%, P < 0.005). Among the plasma lipids, only triglycerides were signi...

In vitro interactions of purified lipoprotein lipase with plasma from type IV hyperlipidemic and normolipidemic subjects were studied using an automated cholesterol analysis adaptation to single vertical spin centrifugation. Lipolysis for 60 min of normolipidemic plasma (control) supplemented with autologous very low density lipoprotein (VLDL) resulted in a progressive conversion of VLDL to low...

Lecithin:cholesterol acyltransferase (LCAT) and cholesteryl ester (CE) transfer activity accumulate linearly in the recirculated medium of the isolated perfused rabbit liver. The appearance of both activities in the perfusate was blocked by the addition of 10 microM colchicine, indicating that these two proteins are synthesized and secreted by the liver. CE transfer activity catalyzed the net t...