In this work, focused on possible application of α-L-fucosidases from bacterial sources in the synthesis of α-L-fucosylated glycoconjugates, several nonpathogenic aerobic bacterial strains were screened for α-L-fucosidase activity. Among them Paenibacillus thiaminolyticus was confirmed as a potent producer of enzyme with the ability to cleave the chromogenic substrate p-nitrophenyl α-L-fucopyra...

We evaluated the clinical usefulness of a recently developed semi-automated one-step chromogenic equivalent of activated partial thromboplastin time (APTT; Behring). This simple test is easily adaptable for automation. Generally, the results with this chromogenic one-step APTT were at least as precise as those obtained with comparative coagulometric methods. The chromogenic one-step APTT showed...

Extensive efforts have been made to develop anti-HIV drugs from potential HIV-1 protease inhibitor1'2) since the protease inhibitors have been proven to produce immature and non-infectious viruses3'4). In order to find HIV-1 protease inhibitors and study the kinetics of inhibition of the protease, many assay methods have been devised using the protease produced by genetic engineering techniques...

To clarify the action of dextran sulphate, a heparin analogue, in the clotting of fibrinogen by thrombin, determinations were carried out on the clotting activity, the release of fibrinopeptides from fibrinogen, and the hydrolytic activity of thrombin against a peptide chromogenic substrate in the absence or presence of antithrombin III (heparin cofactor). It was shown that dextran sulphate its...

The present investigation was designed to evaluate the effect of the antimicrobial agent chlorhexidine on proteolytic degradation. Chlorhexidine, at a final concentration of 0.01%, significantly (p = 0.05) affected the degradation of the general chromogenic substrate azocoll by both commercial proteases and cell-bound bacterial proteases. Reduction of proteolytic degradation was observed follow...

In this new photometric assay fecal samples are pretreated with detergent and high concentrations of salts. The subsequent kinetic enzyme determination step involves the chromogenic substrate succinyl-Ala-Ala-Pro-Phe-4-nitroanilide. The sample pretreatment assures a nearly complete solubilization of the formerly particle-bound enzyme, thus permitting determination of the enzyme activity in eith...

By using a chromogenic substrate for an ubiquitous lysosomal enzyme, hexosaminidase to estimate cell numbers, a sensitive and simple procedure has been developed in which microtiter reaction wells are directly scanned in a spectrophotometer. This method has been adapted to several cell biological assays. Quantitation of the biological activities of T cell growth factor and interferon can be per...

ASSAY PRINCIPLE: Antithrombin is the major physiological coagulation inhibitor. It inhibits coagulation serine esterases, especially Thrombin, Factor Xa and Factor IXa, regulates coagulation pathway and prevents from thrombosis. In the absence of heparin, Antithrombin is a “slow acting”, progressive, inhibitor of coagulation serine esterases. The antithrombin Anti-IIa method is a method based o...