DNA stable-isotope probing (DNA-SIP) is a powerful technique for identifying active microorganisms that assimilate particular carbon substrates and nutrients into cellular biomass. As such, this cultivation-independent technique has been an important methodology for assigning metabolic function to the diverse communities inhabiting a wide range of terrestrial and aquatic environments. Following...

The sexual inducer of Volvox carteri f. nagariensis is a glycoprotein and one of the most potent biological effector molecules known. It is synthesized by sperm cells and converts asexually growing males and females to the sexual pathway. Until now, large-scale production of the inducer was made impossible by an inherent biological ;switch' mechanism, the spontaneous self-induction of asexually...

Previously, we reported antibacterial activity in the body fluid of the nematode Ascaris suum (Kato, Y. (1995) Zool. Sci. 12, 225-230). The antibacterial activity is due to a heat-stable and trypsin-sensitive molecule that was designated as ASABF (A. suum antibacterial factor). In the present study, the purification, determination of primary structure, and cDNA cloning of ASABF were carried out...

Previous studies have shown that the small subunit of Xenopus DNA polymerase gamma (pol gammaB) acts as a processivity factor to stimulate the 140 kDa catalytic subunit of human DNA polymerase gamma. A putative human pol gammaB initially identified by analysis of DNA sequence had not been shown to be functional, and appeared to be an incomplete clone. In this paper, we report the cloning of ful...

The apical membrane antigen 1 (AMA1) has emerged as a promising vaccine candidate against malaria. Advanced evaluation of its protective efficacy in humans requires the production of highly purified and correctly folded protein. We describe here a process for the expression, fermentation, refolding, and purification of the recombinant ectodomain of AMA1 (amino acids 83(Gly) to 531(Glu)) of Plas...

A low molecular weight polypeptide with multiplication-stimulating activity (MSA) was isolated from serum-free media conditioned by a clone of normal Buffalo rat liver cells. The purification of MSA was achieved by gel permeation chromatography of the acid-soluble growth-promoting activity on Bio-Gel P-10 in 1 M acetic acid and followed by reversed phase high pressure liquid chromatography on m...

AcDNA clone (PLGSTA) of 896 bp, containing an open reading frame encoding a 225-amino-acid polypeptide of M, 25 723, was isolated from a cDNA library constructed in Agtl 1 from the liver of a marine teleost flatfish, the plaice (Pleuronectes platessa). The identification of this cDNA as that coding for the subunit of the major cytosolic glutathione S-transferase of plaice liver, GST-A, was supp...

in a recent paper, de baets et al. have characterized the fuzzytolerance and fuzzy equivalence relations that a given strict order relation iscompatible with. in this paper, we generalize this characterization by consideringan arbitrary (crisp) relation instead of a strict order relation, while payingattention to the particular cases of a reflexive or irreflexive relation. the reasoninglargely ...

to assess the microbiological status of a biofiltration system used to treat borehole water, filtermatrix samples were analyzed after staining for the presence of active biofilms using confocal laser scanningmicroscopy (clsm). clsm revealed the presence of biofilms on the filter matrix with actively metabolizingmicrobial cells present. thereafter, heterotrophs,manganese oxidizing bacteria (mob)...