A competitive PCR technique was used to enumerate the proteolytic bacterium Clostridium proteoclasticum from the rumen. A PCR primer, which circumscribes this organism and several closely related strains, was designed for a variable region within their 16S rRNA genes and was used in conjunction with a universal forward primer. This primer pair was tested for specificity against 85 ruminal bacte...

Formation of serine and pyruvate as intermediates in uric acid fermentation by Clostridium acidi-urici was suggested by Radin and Barker (1953), Sagers and Beck (1956), and Rabinowitz and B3arker (1956) to account for the labeling of acetate formed from C14-labele(d purines, and from the ability of cell suspensions and extracts to deaminate serine to pyruvate and ammonia. The present communicat...

was able to obtain large volumes of high potency material by batch cultivation in tanks, but did not report any yields. was sterilized at 118 to 122 C for 1 hr, cooled rapidly to 36 to 39 C, and inoculated immediately. Seed cultures of Clostridium tetani (Massachusetts strain) were maintained at -70 C in 1.0-ml amounts in glass ampoules. One or four of these ampoules were thawed and inoculated ...

Fragment C is the C-terminal domain of the heavy chain of tetanus toxin that can promote the immune response against the lethal dose of this toxin. Therefore, this portion can be considered as a candidate vaccine against tetanus infection, which occurs by Clostridium tetani. The present study aimed to compare the expression of tetanus toxin fragment C in Escherichia coli  BL21 (DE3) pLysS cells...

Eukaryotic F-actin is constructed from two protofilaments that gently wind around each other to form a helical polymer. Several bacterial actin-like proteins (Alps) are also known to form F-actin-like helical arrangements from two protofilaments, yet with varied helical geometries. Here, we report a unique filament architecture of Alp12 from Clostridium tetani that is constructed from four prot...

A series of pyrano[2,3-c]pyrazole derivatives of indole was synthesized by multi-component reactions using the conventional and microwave irradiation approach. Particularly valuable features of this method include high yield, broad substrate scope, shorter reaction times and straightforward procedure. Antimicrobial screening of the synthesized derivatives against eight human pathogens, namely B...