In skin and hair research drug targeting to the hair follicle is of great interest. Therefore the influence of permeant lipophilicity and vehicle composition on local accumulation has been examined using confocal laser scanning microscopy (CLSM). Formulations saturated with either Oregon Green 488, Bodipy FL C(5) or Bodipy 564/570 C(5) were prepared. The dyes were applied in citric acid buffer,...

Although Marvin Minsky invented the basic principles as early as in 1955, it took several decades until confocal laserscanning microscopy (CLSM) finally entered the centre stage of zoological research. However, while CLSM datasets per se contain three-dimensional information, merged projection images resulting from confocal stacks provide a representation of the respective signal in 2 dimension...

To explore the potential of combining non-contact profilometry (NCP) and confocal laser scanning microscopy (CLSM) data to measure entire erosive process non-destructively validate findings using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), electron (SEM) surface microhardness (SMH) same samples throughout. Polished bovine enamel (n = 35) were divided into groups (7/group)...

The backfill quality of a pipeline has an important influence on operation. When loose is used, the may be damaged after short term In this study, numerical simulation analysis buried pipes was carried out under three conditions: around pipe, dense backfill, and controlled low strength materials (CLSM) backfill. effects narrow trench backfilling using CLSM force deformation pipelines were studi...

Dinoflagellates nuclei allow for liquid crystalline characterization without core histones. In this study, nuclei were isolated from the athecate Karenia dinoflagellate species with minimum destruction to their native structure during preparation procedures. The liquid crystalline nuclei were studied by microscopy techniques of Metripol birefringence microscopy, Confocal Laser Scanning Microsco...

Due to the higher resolution, confocal microscopy (CLSM) can be applied to refine the origin of tiny structures of the autofluorescent exoskeletons of microarthropods (mites in particular) which are hard to visualize using traditional differential interference contract light microscopy (DIC LM) and phase contrast light microscopy (PC LM). Three-dimensional (3D) reconstructions of the prodorsal ...

This study was designed to identify surface and subsurface microscopic changes in different carious lesions by using Confocal Laser Scanning Microscope (CLSM) and Image analyzer (light microscopy). Thirty extracted carious posterior teeth were fixed, embedded and polymerized in plastic fixation medium. The final thin sections (80mm) were stained with H&E and Masson Goldner's Tricome while other...

The efficacy of heterogeneous catalysis relies heavily on diffusion and distribution reactants within catalyst supports. However, the presence confinement, essential for reaction selectivity, drastically slows down molecular transport. Here, macro-mesoporous silica inverse opal (SiO2?IO) films were used as a model system to study rather unexplored infiltration behavior using probe molecule rese...

We have implemented and tested a new automatic method for the montage synthesis and 3D reconstruction of large tissue volumes from confocal laser scanning microscopy data (CLSM). This method relies on maximization of the phase correlation between adjacent images. It was tested on a large specimen (a murine heart) that was cut into a number of individual sections with thickness appropriate for C...

A novel method to monitor in situ hydrolyzable casein fragments during cheese ripening by using immunofluorescent labeling and confocal laser scanning microscopy (CLSM) was developed. Monoclonal single chain variable fragments of antibody (scFvs) were generated by antibody phage display toward three small synthetic peptides of the alpha(s1)-casein sequence. These peptides traverse enzymatic cle...