Methylmalonyl-CoA epimerase (MCE) converts d-methylmalonyl-CoA epimer to l-methylmalonyl-CoA epimer in the propionyl-CoA to succinyl-CoA pathway. Only seven cases of MCE deficiency have been described. In two cases, MCE deficiency was combined with sepiapterin reductase deficiency. The reported clinical pictures of isolated MCE are variable, with two asymptomatic patients and two other patients...

Abstract The wax layer of apple leaves plays an important role in improving stress resistance, but relatively little is known about the mechanisms synthesis and transport leaves. In this study, 17 components, including alcohols, alkanes, fatty acids terpenes, were analyzed by gas chromatography-tandem mass spectrometry (GC-MS) from 123 germplasms. Whole-genome sequencing these accessions yielde...

Engineering metabolic biosynthetic pathways has enabled the microbial production of many useful chemicals. However, pathway productivities and yields are often limited by metabolic imbalances. Synthetic regulatory circuits have been shown to be able to balance engineered pathways, improving titers and productivities. Here we developed a negative feedback regulatory circuit based on a malonyl-Co...

One hundred and ninety eight samples of human and animal sources from different areas in El-Sharkia Governorate were used in this study to isolate Staphylococcus species and to determine the presence of coagulase ( coa) gene,a virulence gene detected in S. aureus (CoPS), by PCR. Primary isolation on mannitol salt agar, $-haemolysis, tube coagulase test (TCT) and other biochemical characterizati...

Background Aortic Coarcatation (CoA) is one of the congenital heart diseases with the rate of 5-8% of Coronary heart diseases(CHDs). Balloon angioplasty is now one of the effective way of treatment for CoA, native or Re-coarctation (Re-CoA). We aimed to assess the immediate, and short term response to angioplasty and stenting, and also complications. Materials and Methods Balloon angioplasty wi...

The gene encoding phenylacetyl-CoA ligase (pcl), the first enzyme of the pathway involved in the aerobic catabolism of phenylacetic acid in Pseudomonas putida U, has been cloned, sequenced, and expressed in two different microbes. In both, the primary structure of the protein was studied, and after genetic manipulation, different recombinant proteins were analyzed. The pcl gene, which was isola...

The human intestinal Eubacterium sp. strain VPI 12708 has been shown to have a multistep biochemical pathway for bile acid 7alpha-dehydroxylation. A bile acid-inducible operon encoding 9 open reading frames has been cloned and sequenced from this organism. Several of the genes in this operon have been shown to catalyze specific reactions in the 7alpha-dehydroxylation pathway. The baiF gene from...