Collagen protein is obtained from fresh pigskin through hydrolysis with trypsin. By further adding extracts from scutellaria baicalensis (a Chinese herb), glutaraldehyde and etc., a novel type of collagen burn-healing membrane can be prepared. This novel type of membrane has a porous cross-linked netlike structure. The degree of crosslinking and mechanical properties of the membrane are examine...

Epidermal cells from adult guinea pig skin attach and differentiate preferentially on substrates of type IV (basement membrane) collagen, compared to those of types I--III collagen. In contrast, guinea pig dermal fibroblasts attach equally well to all four collagen substrates. Fibronectin mediates the attachment of fibroblasts but not of epidermal cells to collagen.

PURPOSE This study was performed to evaluate the periodontal wound healing effect of particulate equine bone mineral on canine alveolar bone defects. METHODS Twelve adult male beagle dogs were used as study subjects. The mandibular second and fourth premolars were extracted prior to the experimental surgery, and the extraction sites were allowed to heal for 8 weeks. After periodontal probing,...

Breast cancer progression involves genetic changes and changes in the extracellular matrix (ECM). To test the importance of the ECM in tumor cell dissemination, we cultured epithelium from primary human breast carcinomas in different ECM gels. We used basement membrane gels to model the normal microenvironment and collagen I to model the stromal ECM. In basement membrane gels, malignant epithel...

Usherin is a basement membrane protein encoded by the gene associated with Usher syndrome type IIa, the most common deaf/blind disorder. This report demonstrates a specific interaction between type IV collagen and usherin in the basement membrane, with a 1:1 stoichiometry for binding. Genetic and biochemical approaches were used to explore the role of type IV collagen binding in usherin functio...

Using serial sections of frozen and AFA-fixed tissues from 34 breast cancers, we studied the presence of basement membrane material in the areas of elastosis. Various amounts of type IV collagen but not of laminin were demonstrated in areas of periductal elastosis. In some tumors, type IV collagen accumulated beneath the basement membrane. Periductal elastosis in areas of extensive fibrosis sho...

Aim: The study aimed to evaluate the clinical outcomes of bone augmentation with a mixture cortico-cancellous allograft and xenograft collagen membrane in horizontal knife-edge alveolar crests.
 Material Method: Patients ridge thickness less than 4 mm by preoperative tomography were included study. Twelve patients (10 Female, 2 Male) treated Cortico-Cancellous Allograft Bovine Bone Mineral...

This study proposes a microfluidic system comprising channel and cylindrical hole for holding commercially available culture insert with collagen vitrigel membrane. Because the bottom surface of top are in same plane, flow medium can be directly applied to cells on surface. Additionally, is easily removable from various biological analyses standard protocol, making this accessible users who not...

A pepsin-resistant triple helical domain (chain 50,000 Mr) of type VIII collagen was isolated from bovine corneal Descemet's membrane and used as an immunogen for the production of mAbs. An antibody was selected for biochemical and tissue immunofluorescence studies which reacted both with Descemet's membrane and with type VIII collagen 50,000-Mr polypeptides by competition ELISA and immunoblott...

Formation, maturation, stabilization, and functional efficacy of the neuromuscular junction (NMJ) are orchestrated by transsynaptic and autocrine signals embedded within the synaptic cleft. Here, we demonstrate that collagen XIII, a nonfibrillar transmembrane collagen, is another such signal. We show that collagen XIII is expressed by muscle and its ectodomain can be proteolytically shed into t...