A haemolytic assay was used to test the complement fixation ability of 16 serum samples with high concentrations of anti-cardiolipin antibodies. Fourteen patients had clinical complications usually associated with these antibodies--namely, recurrent abortions, thrombosis, or thrombocytopenia. Complement fixation by anticardiolipin antibodies was shown in only four of these patients and was not ...

Complement fixation titers of 1:2 and 1:4 with coccidioidin are not always of diagnostic significance. The concurrent use of the complement fixation and immunodiffusion tests is an effective means for specific serologic diagnosis of coccidioidomycosis in patients with low levels of complement-fixing antibodies. In routine testing, it is recommended that only sera that are negative for complemen...

A method for the Reiter protein complement-fixation test using the Techncon AutoAnalyzer is described. Two hundred and fifty sera were tested, when six false-positive and nine false-negative results were obtained when compared with results obtained by the manual method. Deep freezing the sera before testing on the AutoAnalyzer increased the sensitivity so that in a further 250 sera tested no fa...

A complement-fixing antigen has been developed, using as source of material CNS tissue from newborn mice infected with the newborn mouse-adapted strain of the Lansing type, MEF1 virus. With this antigen, specific reactions have been obtained with sera from mice, cotton rats, and monkeys immunized with the Lansing-type virus, and from monkeys and chimpanzees convalescent from infection with this...

Although the neutralization test is, in general, satisfactory for immunologic studies with a diversity of viruses, it is cumbersome and expensive. The complement fixation test, in contrast, is simple and rapid and has been successfully applied to a number of viral diseases, especially for diagnostic purposes. In the case of herpes simplex virus infections, however, the literature concerning the...

A report by Bedson (1950) had not shown such an association, nor was any such relationship noted between non-specific urethritis and the results of the complement-fixation test for enzootic abortion in ewes by Willcox and Stamp (1953). In the latter investigation some 323 complementfixation tests for enzootic abortion in ewes were performed on sera from 123 patients with present or past non-spe...

It is well known that Tsutsugamushi disease or scrub typhus in Japan has been prevalent in a limited northern district of the country, but Japanese investigators were not aware that there was a different type of scrub typhus in other parts of Japan, although some sporadic cases had been reported in the foothills of Mt. Fuji (Sakita, 1934), and Kanagawa Prefecture (Fukuzumi et al., 1950), which ...

An automated method to detect TrypanosomÐ crozi antibodies is described, using complement fixation with an adequate automated equipment. Fifty five serum samples from patients lvhere Trypanosoma cruzi antiþodies were detected by Almeida's technique for complement fixation and by fndirect Immunofluorescence test, were studied by this method. The conclusion is that this automated üechnique can be...

The complement-fixation reaction with gonococcal antigen (gonococcal complement-fixation test: GCFI) is based on the same serological principle, and has been known for about as long, as the Wassermann reaction. Up to the 1940's the GCFT was widely employed, but as improvements in cultivation techniques for gonococci were developed, interest in the complement-fixation reaction diminished. At pre...

The serological diagnosis of virus infections depends for the most part on neutralization and complement fixation tests. Both of these have been described in the case of herpes simplex infections, but the neutralization test is used almost exclusively at present, owing to the conflicting results obtained by complement fixation. The reports in the literature on this subject are at variance with ...