partially fed (4 -5 days) h .a. anatolicum (iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (gs), gut membrane antigen (gm), triton extracted gut membrane antigen ( tx-gm ) and sodium dodecyl sulphate treated triton extracted gut membrane antigen (sds-tx-gm). the new zealand white rabbits were immunized with gs, gm, tx- gm...