A gold nanoparticle-based competitive colorimetric assay uses the ensemble of Concanavalin (ConA) and mannopyranoside-encapsulated gold nanoparticles (Man-GNPs) to identify the binding partners for ConA and the binding constants are determined based on the wavelength shifts.

We describe a simplified method for purifying radiolabeled glycopeptide hormones (thyrotropin, lutropin, follitropin, and human choriogonadotropin), which results in preparations of over 95% immunoreactivity. This method is based on competitive elution from columns of the plant lectin, concanavalin A, and is applicable to glycopeptide hormones derived from several species.

In this study, we demonstrate that both highly purified T4+ and T8+ lymphocytes can produce substantial amounts of Interleukin 2(IL 2) when stimulated with the combination of concanavalin A (Con A) and phorbol myristate acetate. Furthermore, addition of IL 1 to macrophage-depleted T lymphocytes significantly increased IL 2 production by lymphocytes of either the T4+ or T8+ phenotype. These find...

The Immunogenicity of γ-irradiated tachyzoites of a highly virulent strain of Toxoplasma gondii (RH strain) was investigated. Immunized mice survived challenge infection and displayed increased interferon gamma (INF-γ ) production. On the other hand, nonimmunized controls died after 10 days and displayed a significantly decreased lymphoproliferative response to Concanavalin A (Con A). No tachyz...

The immunochemical techniques of quantitative precipitation and hapten inhibition have been employed in a study of the interaction of concanavalin A with several.levans of bacterial and plant origin. Of the polysaccharides that form a precipitate with concanavalin A (dextrans, D-mannans, glycogens, amylopcctins, and levans), levans appear to be the least reactive. Hapten-inhibition studies were...

The dielectrophoretic (DEP) behavior of individual yeast cells (5-7 microm in diameter) in aqueous media was observed in a fabricated planar quadrupole microelectrode with a working area of 100 microm in diameter by an optical microscope. The yeast cells migrated in the radial direction in the working area. The DEP velocity of the cells increased as they approached the electrode. The DEP trajec...

B-decachloro-o-carborane derivatives in which one of the carbon atoms was substituted by -CH2CH2CO2H (I), -CH2CHOHCH2-O-CH2CH=CH2 (II) and -CH2CHOHCH2-O-p-C6H4NHCOOC(CH3)3 (III) were prepared from decachloro-o-carborane and the corresponding bromo (I) or epoxi (II and III) derivatives under alkaline conditions. II could be epoxidized and bound to dextran, Concanavalin A, and human IgG, with a b...

Purification of proteins on a large scale is a complex multistep process, and alternative economic strategies are required. This study presents a novel approach (Affinity Sinking, AS) for purification of native proteins utilizing nonimmobilized modified ligands. The nonimmobilized state of the ligand circumvents the need for immobilizing ligands to polymeric supports. Therefore, purification fr...

We have investigated the relationship of concanavalin. A binding to the cell surface of normal and transformed cells and the subsequent agglutination of the transformed cells. At room temperature almost no differences could be detected in agglutinin binding between transformed and untransformed cells. At 0 degrees C, however, where endocytosis was negligible, the transformed cells bound three t...

Ferritin was purified 33,000-fold from the plasma of patients with idiopathic hemochromatosis. The plasma ferritin was labeled with 131I and injected into 2 normal men. Clearance was found to be relatively slow, with 50% 131I-ferritin remaining in the plasma at 27-30 hr. The fraction of plasma ferritin that bound to concanavalin-A was found to be cleared more slowly than the nonbinding fraction...