background: p. atrosepticum is a commercially important pathogen. it causes blackleg in the field and soft rot of tubers after the harvest. this effect is due to secretion of depolymerases and other virulence factors by several mechanisms including t3ss objectives: the effect of bacterial t3ss on solanum tuberosum (s. tuberosum) varieties and its relationship with s. tuberosum resistance gene e...

The aacA29b gene, which confers an atypical aminoglycoside resistance pattern to Escherichia coli, was identified on a class 1 integron from a multidrug-resistant isolate of Pseudomonas aeruginosa. On the basis of amino acid sequence homology, it was proposed that the gene encoded a 6'-N-acetyltransferase. The resistance gene was cloned into the pET23a(+) vector, and overexpression conferred hi...

The present study analyzed the prognostic impact of MET gene copy number in patients with curatively resected gastric cancer who received a combination regimen of cisplatin and S-1. The MET gene copy number was analyzed by use of quantitative real-time polymerase chain reaction. From January 2006 to July 2010, 70 tumor samples from 74 patients enrolled in a pilot study were analyzed. According ...

Gene silencing in plants using topical dsRNA is a new approach that has the potential to be sustainable component of agricultural production systems future. However, more research needed enable this technology as an economical and efficacious supplement current crop protection practices. Systemic gene one key enabling aspect. The objective was better understand topically-induced, systemic trans...

Cells integrate nutrient sensing and metabolism to coordinate proper cellular responses to a particular nutrient source. For example, glucose drives a gene expression program characterized by activating genes involved in its metabolism, in part by increasing glucose-derived histone acetylation. Here, we find that lipid-derived acetyl-CoA is a major source of carbon for histone acetylation. Usin...

Pyruvate kinase M2 (PKM2) and pyruvate dehydrogenase complex (PDC) regulate production of acetyl-CoA, which functions as an acetyl donor in diverse enzymatic reactions, including histone acetylation. However, the mechanism by which the acetyl-CoA required for histone acetylation is ensured in a gene context-dependent manner is not clear. Here we show that PKM2, the E2 subunit of PDC and histone...