A prospective study of 12 families in which the proband had Fuchs's dystrophy was undertaken. Forty-four relatives were examined with the clinical specular microscope. Nine relatives, all women over 40 years of age, were affected. The mean endothelial cell density for unaffected relatives was 2889 cells/mm2 in the right eye and 2923 cells/mm2 in the left eye. Variability in endothelial cell siz...

PURPOSE Human corneal endothelial cell (HCEC) density decreases with age, surgical complications, or disease, leading to vision impairment. Such endothelial dysfunction is an indication for corneal transplantation, although there is a worldwide shortage of transplant-grade tissue. To overcome the current poor donor availability, here we isolate, expand, and characterize HCECs in vitro as a step...

Purpose. To compare the loss of corneal endothelial cells after phacoemulsification according to different anterior chamber depths (ACDs). Methods. We conducted a prospective study on 135 eyes with senile cataracts. Eyes with nuclear density grades of 2 to 4 were divided into three groups according to ACD: ACD I, 1.5 < ACD ≤ 2.5 mm; ACD II, 2.5 < ACD ≤ 3.5 mm; or ACD III, 3.5 < ACD ≤ 4.5 mm. In...

BACKGROUND The nature of chronic endothelial cell loss in homologous corneal grafts is still unclear. Possible causes are cell migration to the recipient bed and chronic subclinical immune reaction. OBJECTIVES To compare endothelial cell loss after autologous rotational keratoplasty and homologous keratoplasty and present the clinical outcome of patients after rotational keratoplasty. METHO...

PURPOSE To evaluate short-term changes in central corneal endothelial cell density and morphology after photorefractive keratectomy (PRK) with mitomycin-C (MMC) 0.02% in patients with moderate myopia. METHODS In this prospective interventional case series, patients with moderate myopia (spherical equivalent refractive error from -4.0 to -8.0 D) underwent PRK with a single intraoperative appli...

PURPOSE To develop a novel method for constructing a sheet of human corneal endothelial cells (HCECs) and examine the properties of the HCEC sheet. METHODS HCECs were cultured on a cell culture insert for a week; ethylenediamine tetraacetic acid was applied from the bottom of the cell culture insert to attenuate the attachment of HCECs. The sheet of HCECs was constructed by bluntly detaching ...

OBJECTIVE To compare the efficacy and safety of the intraocular lens (IOL)-shell procedure versus conventional phacoemulsification for the surgical treatment of dense cataracts. METHODS Eighty eyes with dense nuclear cataracts were enrolled in a prospective, randomized controlled study. Patients were assigned to two groups. In Group I, the IOL was traditionally implanted after all nuclear fra...

PURPOSE To study the endothelial cell morphology and corneal thickness changes after phacoemulsification by using the OZil torsional and longitudinal ultrasound techniques (Infiniti Vision System, Alcon Laboratories). SETTING Department of Ophthalmology, Clinical Center, University of Debrecen, Debrecen, Hungary. METHODS 52 patients with cataract were randomly assigned to longitudinal ultra...

PURPOSE To compare the ability of different ophthalmic viscoelastic devices to protect the corneal endothelium following in-the-bag phacoemulsification with posterior chamber intraocular lens (IOL) implantation. MATERIAL & METHODS We studied 50 patients with soft to moderately dense (Grade 1-3) cataract and corneal endothelial cell density of >2000 cells/mm2. The corneal response to surgery w...

BACKGROUND Descemet's stripping automated endothelial keratoplasty (DSAEK) surgery offers a more standardized approach and reliable method to create corneal grafts with an instrument such as a microkeratome. With the development of Descemet's membrane endothelial keratoplasty, an excellent clinical outcome is seen in the treatment of corneal endothelial dysfunctions, which indicates that thinne...