A great deal of an elicitor-active oligosaccharide (1.47 g) was obtained from 40 g of polysaccharide laminaran with the guidance of elicitor activity in the bean cotyledon assay. Physicochemical means suggested that the oligosaccharide was a beta-1,3- and beta-1,6-linked triglucoside. This was a partial structure of LN-3, having elicitor activity to alfalfa and bean. The triglucoside exhibited ...

Ammonium sulfate chromatography has been employed to separate glyceraldehyde 3-phosphate dehydrogenases (GPD) of Sinapis alba cotyledons of various developmental stages. Cotyledons of dark-grown seedlings possess one major NAD-specific enzyme designated NAD-GPD I. Irradiation with continuous far red light leads to a strong increase in NADP-GPD activity and to the formation of a second NAD activ...

Different varieties of peas have different proportions of rDNA in their genomes; there is no obvious correlation between the proportions and seed or leaf size. The rDNA proportions in root tips, seedlings, leaves and in the cotyledon cells of high DNA content, were compared in four varieties. In three, there was no difference between tissues; the fourth showed an amplification of rRNA genes in ...

The cucumber cotyledon greening bioassay is frequently used for detecting cytokinins. Beneficial modifications of the original technique included using 5-day-old cucumber (Cucumus sativus L., cv. National Pickling) cotyledons treated with combinations of 40 millimolar KCl and various concentrations of cytokinins. A dark incubation period of 20 hours was followed by an exposure to light for 3.5 ...

DORNROSCHEN (DRN), which encodes a member of the AP2-type transcription factor family, contributes to auxin transport and perception in the Arabidopsis embryo. Live imaging performed with transcriptional or translational GFP fusions shows DRN to be activated in the apical cell after the first zygotic division, to act cell-autonomously and to be expressed in single cells extending laterally from...

This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed. To purify spermatozoa, motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa and placental cotyledon, as a positive control, and then...

The accumulation of tetraphenylphosphonium (TPP(+)), 5,5'-dimethyl-oxazolidine-2,4-dione (DMO), and a micro pH electrode were used to measure membrane potential, intracellular and extracellular pH, respectively, upon the addition of exogenous sucrose to soybean cotyledon protoplasts. Addition of sucrose caused a specific and transient (a) depolarization of the membrane potential (measured by TP...

The distribution of napin and cruciferin, the two major storage proteins in rape seed, Brassica napus, has been visualized during seed development by antibody staining of paraffin-embedded and sectioned seeds. The results indicate that the synthesis of both proteins during embryogenesis is strictly regulated with respect to time and tissue. Although the synthesis of napin started a few days ear...

Somatic embryogenesis is an efficient micro-propagation technique, which can lead to mass multiplication of tea with a tap root. Hence, the development viable somatic and protocol through liquid culture conditions needed increase efficiency protocol. Cotyledon-derived embryos, leaf stem callus TRI 5001 cultivar, were tested different MS media. A significantly higher cotyledon-derived embryo rat...