We introduce a new method for purifying recombinant proteins expressed in bacteria using a highly specific, inducible, self-cleaving protease tag. This tag is comprised of the Vibrio cholerae MARTX toxin cysteine protease domain (CPD), an autoprocessing enzyme that cleaves exclusively after a leucine residue within the target protein-CPD junction. Importantly, V. cholerae CPD is specifically ac...

A procedure was developed to prepare in large amounts two carboxypeptidases, CPD-I and CPD-II, from Aspergillus niger. They were each shown to be serine proteases and single-chain monomers with molecular masses of ca. 81 kDa and containing 22% carbohydrates. Amino acid analysis, carbohydrate determination, and N-terminal sequencing (20 to 25 residues) were performed on each enzyme. CPD-I showed...

Synthetic Environments (SE) facilitate an easy setup of various virtual realities since they are component-based and relatively easy maintainable. Through three stages, involving various types of stakeholders, the feasibility of SEs for cooperative product design (CPD) was explored: 1) Semi-structured group interviews with 19 engineers and designers assessed SE’s potential for CPD; 2) Implement...

Membrane-bound carboxypeptidase D (CPD) is a B-type carboxypeptidase that specifically cleaves C-terminal Arg or Lys from peptides and proteins. RAW 264.7 cells contained significant membrane-bound CPD activity as shown by activity assays and immunoprecipitation. To determine whether CPD can increase nitric oxide (NO) synthesis by releasing precursor Arg, cells were activated in Arg-free medium...

We give a precise characterization of two important classes of conditionally positive definite (CPD) kernels in terms of integral transforms of infinitely divisible distributions. In particular, we show that for any stationary CPD kernel A(x, y) = f(x − y), f is the log-characteristic function of a uniquely determined infinitely divisible distribution; further, for any additive CPD kernel A(x, ...