Using in situ hybridization, individual cells expressing mRNAs for collagen types I and IV were localized in fixed-tissue sections of adult and fetal human optic nerve heads. Astroglial cells lining the cribriform plates and cells inside the cribriform plates of the lamina cribrosa had mRNA for collagen type IV. Cells in the glial columns, pial septa, and vascular wall also contained mRNA colla...

Adenoid cystic carcinoma (ACC) may acquire a chemokine-mediated mechanism during the process of metastasis. To investigate the involvement of chemokines in metastasis from ACC, expression of CXCR4 in surgical specimens of ACC and two tumor lines transplantable to nude mice was examined immunohistochemically. In addition, the expression levels of CXCR4 protein and mRNA were examined by Western b...

Objective: To characterize the mammographic appearance of cancerization of lobules by ductal carcinoma in situ (DCIS), by correlating imaging and histological findings. Materials and Methods: This retrospective study was based on a review of the histopathological reports of 135 patients who underwent breast biopsy. A diagnosis of cancerization of lobules by DCIS was confirmed in 12 patients. Tw...

BACKGROUND The anatomy of septal defects can be complex and morphologically unpredictable. Balloon sizing of such defects may not be feasible, and an appropriately sized commercial occluder may not be available. Therefore, percutaneous transcatheter closure of such defects can be challenging because of an increased risk of complications. In this study, we have described the efficacy and safety ...

PURPOSE Previous studies have suggested that the lamina cribrosa (LC) and its surrounding sclera are biomechanically important in the pathogenesis of glaucoma, but many were limited by assumptions of tissue isotropy and homogeneity. Here, we used an improved biomechanical model driven by experimental measurements of scleral and LC collagen fiber organization to more accurately evaluate optic ne...

The lamina cribrosa (LC) plays an important biomechanical role in the optic nerve head (ONH). We developed a statistical shape model of the LC and tested if the shape varies with age or IOP. The ONHs of 18 donor eyes (47-91 years, mean 76 years) fixed at either 5 or 50 mmHg of IOP were sectioned, stained, and imaged under a microscope. A 3D model of each ONH was reconstructed and the outline of...

PURPOSE To investigate the biomechanical response of the optic nerve head (ONH) connective tissues to IOP elevation in three pairs of monkey eyes in which one eye had early experimental glaucoma (EG). METHODS A serial imaging technique was used to reconstruct the ONH and peripapillary sclera of three pairs of unilateral EG eyes fixed at 10 mm Hg. Eye-specific finite element models of the post...