Systemic lupus erythematosus (SLE) is known to proceed to clinically relevant nephritis in more than 50% of patients and, in about 20% of these patients, to terminal renal failure. Thus, renal replacement therapy including kidney allografting is required for a considerable number of SLE-patients. For allografting patients’ donor-specific antibodies against HLA molecules of given donors (DSA) ha...

Background. Although blood ordering is a common practice in surgical field, the average requirement for a particular procedure is usually based on subjective anticipation of blood loss rather than on evidence based estimates. Overordering with minimal utilization squanders technical time, reagent and imposes extra expenses on patients. This study was conducted to assess blood utilization practi...

A totally revised crossmatching and antibody screening procedure has been designed using low ionic strength saline as the suspending medium for red cells in the saline and enzyme phases as well as the antiglobulin test. The replacement of Pasteur pipettes with precision samplers for all serum and red cell dispensing has resulted in improvement in the reproducibility and standardisation of techn...

Under current UK standards of deceased donor typing, the formation by the recipient of HLA antibody against an allelic variant of a 'self' antigen creates a particular problem for organ allocation. In the reported case, the decision to transplant was taken in the situation of a positive flow crossmatch result attributed to allelic antibody. The potential that target antigen density contributed ...

The monitoring of the levels of alloantibodies following transplantation might facilitate early diagnosis of chronic rejection (CR), the leading cause of renal allograft failure. Here, we used serial alloantibody surveillance to monitor patients with preoperative positive flow cytometric crossmatch (FCXM). Sixty-nine of 308 renal transplant patients in our center had preoperative positive FCXM....

The overarching goal of Bio 4342/W research over the past several years has been to understand a euchromatic region of the Drosophila virilis genome well enough to be able to distinguish this domain at the DNA level from the heterochromatic counterparts in its genetic relatives, such as Drosophila melanogaster, the common fruit fly. The class will utilize the well-established genome of D. melan...