A water-soluble crude polysaccharide preparation (GFP) was obtained from Grifola frondosa fruit bodies by hot water (100 °C) extraction, followed by ethanol (75%) precipitation. Purified polysaccharides, GFP75-2D was obtained from the crude material by successive fractionation using DEAE-Sepharose Fast Flow ion exchange chromatography and Sephacryl S gel filtration chromatography. GFP75-2D main...

Whole soybeans serve as one of the main sources of protein in ruminant nutrition. Different processing methods have been employed for ruminal protein protection. The present study was conducted to determine the effects of microwave irradiation [900 W; 2, 4 and 6 min] on quality, ruminal degradability and estimated in vitro intestinal digestibility of availability soybean crude protein....

Bidens pilosa is a plant used by Amazonian and Asian folks for some hepatopathies. The hydroethanol crude extract and three fractions were assessed for antioxidant and hepatoprotective effects. Higher levels of scavenger activity on the 1,1-diphenyl-2-picrylhydrazyl radical, inhibition of deoxyribose oxidation and lipid peroxidation in vitro were detected for the ethyl acetate fraction (IC(50)~...

A study was conducted in a completely randomized design to evaluate the effect of diets formulated on the basis of four critical essential amino acids (lysine, methionine, tryptophan and threonine) on the crude protein requirement, carcass quality, nutrient digestibility, haematological and blood biochemical indices of broiler finisher chickens (28-56 days) reared under tropical environment. On...

The aim of investigation was to determine the effects of aspect (north and south) on some morphological [plant height (cm), number of stems per plant, main stem diameter (mm), number of leaves per main stem, petiole length (cm), leaflet length (cm), leaflet width (cm), leaf: stem ratio] and chemical [crude protein (%), crude cellulose (%), calcium (%), phosphorus (%), potassium (%) and magnesiu...

Background: The ethno-medical significance of Clerodendrum genus raises the interest towards the characterization of its seed lectin by inexpensive and most effective technique.Objective: The focus of this study is the purification, characterization, and evaluation of the antioxidant and antiproliferative potential of a galactose-specific lectin from Cler...

Crude cytoplasmic extracts from vesicular stomatitis virus (VSV)-infected HeLa cells incorporate radioactive amino acids into hot trichloroacetic acid-precipitable material linearly for 10 to 20 min. The material synthesized in vitro corresponds in molecular weight to four of the five VSV structural proteins. However, synthesis of the viral glycoprotein (G) is significantly reduced, whereas the...

The component enzymes of the chloroplast-associated, acyl carrier protein (ACP)-dependent, fatty acid synthetase (FAS-11) from Euglena gracilis have been independently examined by gel filtration chromatography of crude extracts from photoautotrophic cells. The acetyl coenzyme A:ACP transacylase, malonyl CoA:ACP transacylase, and /3-ketoacyl-ACP reductase activities were clearly resolved with a...