To understand the impact of a hypovirus infection on the secretome of the chestnut blight fungus, Cryphonectria parasitica, a phytopathogenic filamentous fungus, two-dimensional electrophoresis (2-DE) and isobaric tag for relative and absolute quantitation (iTRAQ) technology were employed to identify and quantify the secreted proteins. A total of 403 unique proteins were identified from the sec...

Targeted disruption of cpg-1, a gene encoding the G protein Gi alpha subunit, CPG-1, in the chestnut blight fungus, Cryphonectria parasitica, results in reduced mycelial growth, reduced orange pigmentation, loss of virulence, loss of asexual sporulation, and female infertility. We report the development of a complementation system for cpg-1 null mutants and its use to evaluate the in vivo conse...

Disruption of the gene encoding Galpha subunit cpg-1 in the chestnut blight fungus Cryphonectria parasitica reduces growth and pigmentation and abolishes reproduction and virulence. We now report the consequences of mutations designed to constitutively activate (Q204-L and R178-C) CPG-1-mediated signaling. Introduction of cpg-1-QL or cpg-1-RC into wild type, Deltacpg-1 and Deltacpgb-1 (Gbeta) m...

Pathogen recognition and transcriptional activation of defense-related genes are crucial steps in cellular defense responses. RNA silencing (RNAi) functions as an antiviral defense in eukaryotic organisms. Several RNAi-related genes are known to be transcriptionally up-regulated upon virus infection in some host organisms, but little is known about their induction mechanism. A phytopathogenic a...

The gene for glyceraldehyde-3-phosphate dehydrogenase (gpd-l) of Cryphonectria parasitica, the chestnut blight fungus, was isolated by probing a genomic library with a PCR (1) amplified copy of the coding region of the Aspergillus nidulans homologue, gpdA (2). Presented below is the nucleotide sequence for the entire gpd-l coding region, 381 bp of the 5'-flanking region and 557 bp of the 3'-fla...

Hypovirulent strain EP713 of the chestnut blight fungus Cryphonectria (Endothia) parasitica harbors a family of viral encoded double-stranded (ds) RNAs thought to be responsible for the hypovirulence phenotype. These include L-dsRNA, described in the accompanying paper (Shapira et al., 1991); several prominent species in the estimated size range of 8 to 10 kb, referred to here as M-dsRNAs; and ...

Efforts to develop American chestnuts [Castanea dentata, (Marsh.) Borkh], with improved resistance the chestnut blight fungus, Cryphonectria parasitica (Murr.) Barr, and Phytophthora root rot, caused by Phythophthora cinnamomi Rands, are ongoing. Establishing founder populations of will require understanding ecological silvicultural requirements species. In areas where white tailed deer (Odocoi...