The development of a reliable method of using PCR for detection of Cryptosporidium oocysts in environmental samples with oligonucleotide primers which amplify a portion of the sequence encoding the small (18S) subunit of rRNA producing a 435-bp product was demonstrated. The PCR assay was found to provide highly genus-specific detection of Cryptosporidium spp. after release of nucleic acids from...

Oocysts of a Cryptosporidium isolate from guinea pigs were not infectious for adult mice, but were infectious for two of three newborn calves and for suckling mice. However, oocysts isolated from calves or mice infected with guinea pig Cryptosporidium were not infectious for guinea pigs. Four isolates of C. parvum from calves were incapable of infecting weanling guinea pigs. Microscopic examina...

introduction: giardia and cryptosporidium are two of the most common parasitic protozoa that cause acute intestinal infections in children, all of the world. these parsites find in water environments, such as untreated water , wastewaters and even treated water. according to, a precise study on surface water was not done, in our country, necessity of precise study by ifa method that is prescise...

A monoclonal antibody enzyme-linked immunosorbent assay (ELISA) was developed to detect Cryptosporidium parvum oocysts in bovine feces. Fecal oocysts were concentrated by centrifugation through Formalin-ethyl acetate solution and captured with monoclonal antibody 18.280.2 reactive with C. parvum oocysts. Captured oocysts were detected with goat anti-oocyst serum, following the addition of a per...

Water samples collected throughout several reclamation facilities were analyzed for the presence of infectious Cryptosporidium parvum by the focus detection method-most-probable-number cell culture technique. Results revealed the presence of infectious C. parvum oocysts in 40% of the final disinfected effluent samples. Sampled effluent contained on average seven infectious oocysts per 100 liter...

Cryptosporidium felis and C. meleagridis in Persons with HIV, Portugal To the Editor: Cryptosporidium, a pathogenic protozoan parasite with a worldwide distribution, causes diar-rheal illness in humans and animals. The parasite can be transmitted from human to human through fecal-oral contact (household contact and noso-comial transmission), sexual contact, ingestion of contaminated food or wat...

Irrigation water and washing water have been inferred to be associated with contamination of fresh fruits and vegetables with pathogenic microorganisms infectious for humans. The objective of the present study was to determine whether apples experimentally contaminated with Cryptosporidium oocysts represent a food safety concern. Laser scanning confocal microscopy revealed no morphological chan...

We reexamined the finding of Neumann et al. that intact Cryptosporidium parvum oocysts obtained after in vitro excystation were infectious for neonatal CD-1 mice. We used both established excystation protocols and our own protocol that maximized excystation. Although intact oocysts isolated after any of three protocols were infectious for neonatal CD-1 mice, the infectivity of intact oocysts is...

Six genera of rotifers including Philodina, Monostyla, Epiphanes, Euchlanis, Brachionus, and Asplanchna were exposed to oocysts of Cryptosporidium parvum cleaned of fecal debris. Unstained oocysts and those stained with fluorescein-conjugated monoclonal antibody were added to suspensions of viable rotifers and were examined by phase-contrast, differential interference contrast, and fluorescence...

ABSTRACT The presence of waterborne pathogens, when associated with the water supply system, poses risks to public health. This study investigated occurrence (oo)cysts Cryptosporidium spp. and Giardia in two Water Treatment Plants, full-cycle technology, assessed microbiological risk consumers’ membrane filtration technique was employed identify protozoan load 24 samples raw filtered water. Phy...