In this study, the potential of electrohydrodynamic atomization or electrospraying to produce nanometer-order CGTase particles from aqueous suspension was demonstrated. CGTase enzyme was prepared in acetate buffer solution (1% v/v), followed by electrospraying in stable Taylor cone-jet mode. The deposits were collected on aluminium foil (collector) at variable distances from the tip of spraying...

In 1968, Kugelberg and Edstr6m described a histochemical method to localize skeletal muscle fibres belonging to a single motor unit by depletion of their glycogen. The authors concluded that "contractions produced striking changes in the phosphorylase activity and glycogen content, which varied in different types of fibres". Thus, the pattern of histochemically demonstrated activity of phosphor...

Glucosyltransferase and glucanotransferase involved in the production of cyclic tetrasaccharide (CTS; cyclo {-,6}-ai-D-glucopyranosyl-(1-,3)-ev-D-glucopyranosyl(1--6)-a-D-glucopyranosyl-(1-3)-a-D-glucopyranosyl(1-,)) from or-1,4-glucan were purified from Bacilltts globisporus Cll. The former was a 1,6-a-glucosyltransferase (6GT) catalyzing the a-1,6-transglucosylation of one glucosyl residue to...

We have cloned the gene that encodes a novel glucosyl transferase (AraGT) involved in rhamnosylation of the polyketide antibiotic Aranciamycin in Streptomyces echinatus. AraGT comprises two domains characteristic of bacterial glycosyltranferases. AraGT was synthesized in E. coli as a decahistidinyl-tagged polypeptide. Purified AraGT is dimeric, displays a T(mapp) of 30 degrees C and can glycosy...