A simple spectroscopic method for determining the cytochrome c oxidase, cytochrome a, a3, content in tissue and mitochondria samples independent of myoglobin or blood contamination is described. Using tissue homogenates solubilized in Triton X-100, this assay relies on the selective reduction of mitochondrial cytochromes by the action of potassium cyanide. Monitoring the optical absorbance of t...

The activities of two mitochondrial enzymes, succinate dehydrogenase and cytochrome oxidase, have been measured by quantitative cytochemistry and microdensitometry in the synoviocytes of rheumatoid and non-rheumatoid synovial lining cells. Although both tended to be higher in the former, there was no statistically significant difference in the activities of either enzyme in these tissues. Howev...

Cytochrome oxidase is a multisubunit, intrinsic membrane protein with a complex function that includes oxidation of cytochrome c, reduction of oxygen and generation of a membrane potential. To clarify the relationship of its normal function to protein and membrane structure, we have examined the kinetic behavior of rat liver cytochrome oxidase in the intact inner mitochondrial membrane and in d...

The 02, CO, and alkyl isocyanide-binding properties of a variety of vertebrate and invertebrate heme proteins have been compared in detail to those of protoheme mOn0-3-( 1-imidazoy1)-propylamide monomethyl ester in aqueous suspensions of soap micelles. The proteins examined include: cytochrome P-450,,, from Pseudomonasputida, beef heart cytochrome c oxidase, yeast cytochrome c peroxidase, a and...

b a ckground: anoph eles fluviatilis , one of the major malaria vectors in iran, is assumed to be a complex of sibling species. the aim of this study was to evaluate cytochrome oxidase i (coi) gene alongside 28s-d3 as a diagnostic tool for identification of an. fluviatilis sibling species in iran. m e t hods: dna sample belonging to 24 an. fluviatilis mosquitoes from different geographical area...