BACKGROUND Free radical injury contributes to cardiac dysfunction during ischemia-reperfusion. Detoxification of free radicals requires maintenance of reduced glutathione (GSH) by NADPH. The principal mechanism responsible for generating NADPH and maintaining GSH during periods of myocardial ischemia-reperfusion remains unknown. Glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme...

MAPK phosphorylation of various substrates is mediated by the presence of docking sites, including the D domain and the DEF motif. Depending on the number and sequences of these domains, substrates are phosphorylated by specific subsets of MAPKs. For example, a D domain targets JNK to c-Jun, whereas a DEF motif is required for ERK phosphorylation of c-Fos. JunD, in contrast, contains both D and...

The adenovirus late IVa2 protein is required for maximally efficient transcription from the viral major late (ML) promoter, and hence, the synthesis of the majority of viral late proteins. This protein is a sequence-specific DNA-binding protein that also promotes the assembly of progeny virus particles. Previous studies have established that a IVa2 protein dimer (DEF-B) binds specifically to an...

We adopt the following convention: x1, x2, z are sets, A is a non empty set, and f , g, h are elements of CA. Let us consider A. The functor +CA yielding a binary operation on CA is defined by: (Def. 1) For all elements f , g of CA holds +CA(f, g) = (+C)◦(f, g). Let us consider A. The functor ·CA yielding a binary operation on CA is defined as follows: (Def. 2) For all elements f , g of CA hold...

Osteogenic disorder shionogi (ODS) rats carry a hereditary defect in ascorbic acid synthesis, mimicking human scurvy when fed with an ascorbic acid-deficient (aa-def) diet. As aa-def ODS rats were shown to feature disordered bone formation, we have examined the bone mineralization in this rat model. A fibrous tissue layer surrounding the trabeculae of tibial metaphyses was found in aa-def ODS r...

Let Γ be a finitely presentable pro-p group such that def(Γ) > 0. If Γ has a nontrivial finitely generated closed normal subgroup N of infinite index then def(Γ) = 1, N is a free pro-p group and Γ/N is virtually free. If π is a finitely presentable group with β (2) 1 (π) = 0 then def(π) ≤ 1, with equality only if c.d.π = 2 or π ∼= Z. (See Theorem 2.5 of [1].) The L-Betti number condition holds ...

The aim of this study was to develop and validate a dissolution test for the quality control of deflazacort (DEF) tablets, a drug mainly used for the prevention of organ transplantation rejection, labeled as containing 6 mg of active pharmaceutical ingredient (API), using an RP-HPLC method. After testing sink conditions and stability at 37°C, DEF was found to be unstable, and decomposed as a fu...

To analyze the effects of a new unknown peptide DEF on the growth of tumor cells, a fused polypeptide TAT-DV1-DEF was designed and synthesized. The lung adenocarcinoma cell line GLC-82 treated with TAT- DV1-DEF was analyzed with a cell counting kit 8, and the location of polypeptides in cells was observed under laser confocal microscopy. The efficiency of polypeptide transfection and changes in...

This paper describes a program representation and algorithms for realizing a novel structural testing methodology that not only focuses on addressing the complex features of object-oriented languages, but also incorporates the structure of object-oriented software into the approach. The testing methodology is based on the construction of contextual def-use associations, which provide context to...

The Australian Defence Standard Def (Aust) 5679, entitled “The Procurement of Computer-Based Safety-Critical Systems”, was originally published in August, 1998. Def (Aust) 5679 is currently undergoing revision. This paper describes the standard and discusses the issues that have been highlighted during the revision process.