Flow cytometric detection of platelet surface-associated IgG (PSAIgG) can be used to determine whether immunologic factors are contributing to thrombocytopenia in dogs. In vitro alterations in platelet activation and morphology, however, could impact the results of this test. The purpose of this study was to determine whether the PSAIgG test for immune-mediated thrombocytopenia was valid on who...

The aim of this study is the evaluation of the e ect of microencapsulation of nanoparticles in composite microparticles on the reduction of burst release. Microparticles (simple and composite) and nanoparticles were prepared by using water-in-oil-in-water (W/O1/W2 double-emulsion solvent di usion/evaporation method), using di erent drug/polymer ratios. For preparation of the composite micropart...

The influence of tobacco-smoke-material (TSM) on the detachment of microparticles from surfaces by turbulent air flow was investigated experimentally. Both clean and dusty glass surfaces were subjected to TSM either before or after the deposition of 64 microm to 76 microm-diameter stainless steel microparticles onto the surfaces. The TSM was generated by mechanically puffing research-grade ciga...

AIMS Microparticles are membrane vesicles shed by cells upon activation and/or apoptosis. Microparticles are involved in several processes, including blood coagulation and thrombosis. In addition to their role in the regulation of lipid metabolism, peroxisome proliferator-activated receptor-γ (PPAR-γ) agonists exert other effects, both dependent on and independent of PPAR-γ activation. Some PPA...

This overview provides information on the current state of endothelial microparticle research. Microparticles are small membrane vesicles shed by different cell types, which contain cell surface proteins and cytoplasmic components of the original cell. The microparticle production is a part of normal cell function, but it increases by apoptotic cells and cells under stress. Numerous evidences s...

BACKGROUND It is well accepted that functional activity of platelet integrin αIIbβ3 is crucial for hemostasis and thrombosis. The β3 subunit of the complex undergoes tyrosine phosphorylation shown to be critical for outside-in integrin signaling and platelet clot retraction ex vivo. However, the role of this important signaling event in other aspects of prothrombotic platelet function is unknow...

A sedentary lifestyle has adverse effects on the cardiovascular system, including impaired endothelial functions. Subjecting healthy men to 7 days of dry immersion (DI) presented a unique opportunity to analyze the specific effects of enhanced inactivity on the endothelium. We investigated endothelial properties before, during, and after 7 days of DI involving eight subjects. Microcirculatory f...

Idiopathic thrombocytopenic purpura (ITP) is an autoimmune disorder. Decreased platelet survival is the main pathogenesis of it, which is related to platelet antibodies (APA). The levels of these antibodies are correlated with relapse and remission which was shown first time by us. Although APA levels decrease in remission but not risappear as studied by us. Among the several approches about IT...

The difference in the blood concentration of tacrolimus between the assay methods, microparticle enzyme immunoassay (MEIA) and enzyme linked immunosorbent assay (ELISA) was observed in a liver transplant recipient with anemia. MEIA provided significantly higher concentration than those of ELISA (7.8+/-1.9 vs. 5.0 +/- 1.8nglml, p<0.05) while the patient had low haematocrit <25%. The difference, ...

t Microparticless are membrane vesicles released from many different cell types. There are two mechanismss that can result in their formation, cell activation and apoptosis. In both these mechanismss different pathways are involved in microparticle generation. Microparticle generationn seems to be a highly regulated process that results in formation of microparticles that varyy in size, phospho...