The serum level of diamine oxidase (DAO) reflects the integrity and maturation small intestinal mucosa. This measure is important in diagnosing various diseases, including chronic urticaria tachyphylaxis, multiple organ dysfunction syndrome, preterm abortion, migraine. review aimed to summarize findings previous studies on changes DAO levels diverse diseases application this enzyme clinical set...

This study is the first to report on the detection of biogenic amines in an aqueous solution using an organic field-effect transistor (OFET) device with an extended gate electrode modified with a layer of diamine oxidase and a horseradish peroxidase osmium-redox polymer. The limit of detection (LOD) for histamine was estimated to be 1.2 μM. These results reveal that extended-gate type OFET devi...

Aminocarboxylate complexes of vanadium(III) are of interest as models for biologically and medicinally relevant forms of this interesting and somewhat neglected ion. The V(III) ion is paramagnetic, but not readily suited to conventional EPR, due to its integer-spin ground state (S=1) and associated large zero-field splitting (zfs). High-frequency and -field EPR (HFEPR), however, has the ability...

AIMS To develop and assess a cytochemical technique for the light microscopical detection of oxidase activity in pathogenic bacteria. METHODS Live bacterial cells were deposited on to aminopropylsilane treated glass coverslips by centrifugation, dried, then reacted with either 1% (w:v) n,n,n',n'-tetramethyl-p-phenylene diamine (TPD) or 5 mM diaminobenzidine (DAB) at 37 degrees C. The preparat...

Neisseria perJava and Hemophilus parainjknzae degrade spermine and spermidine to 1,3-propanediamine during growth (1). One or both of these polyamines are essential growth factors or are growth-stimulatory to a number of microorganisms (2-8), and these compounds occur in many microorganisms (1) and in most mammalian tissues (9). There have been few studies on the enzymatic degradation of polyam...

The amiloride binding protein (ABP) is detected in many epithelium-rich and/or hematopoietic tissues (Lingueglia, E., Renard, S., Voilley, N., Waldmann, R., Chassande, O., Lazdunski, M., and Barbry, P. (1993) Eur. J. Biochem. 216, 679-687). The protein binds amiloride and some of its derivatives, such as phenamil, benzamil, and ethylpropylamiloride. These properties have previously suggested th...

Diamine oxidase (DAO) is abundantly expressed in mammalian small intestine catalyzing the oxidative breakdown of polyamines and histamine. The aim of this study was to determine the relationship between stimulation of intestinal diamine oxidase secretion with intestinal fat absorption and histamine release. Conscious intestinal lymph fistula rats were used. The mesenteric lymph ducts were cannu...